Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7519-7523.doi: 10.3969/j.issn.1673-8225.2010.40.024

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Influence of Thy-1.1 stem cell transplantation on nitric oxide synthase of injured artery

Liu Hua-dong, Dong Shao-hong, Jiang Xin, Luo Lin-jie   

  1. Department of Cardiology, Shenzhen People’s Hospital, Second Clinical College, Jinan University, Shenzhen  518020, Guangdong Province, China
  • Online:2010-10-01 Published:2010-10-01
  • About author:Liu Hua-dong☆, Doctor, Attending physician, Department of Cardiology, Shenzhen People’s Hospital, Second Clinical College, Jinan University, Shenzhen 518020, Guangdong Province, China lhd2578@163.com

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Abstract:

BACKGROUND: At present, effects of stem cell transplantation on restenosis following angiopoiesis are controversial. It is of great significance to explore effects of stem cell transplantation on restenosis and its action mechanism.
OBJECTIVE: To study the effect of local transplantation of rat Thy-1.1 stem cells on endothelial hyperplasia following common carotid artery aortic sac, explore effects of stem cell transplantation on endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS), and evaluate the relationship of restenosis and NOS after stem cell transplantation.
METHODS: Male 4-6 weeks Sprague Dawley rats were used for preparing Thy-1.1 stem cells. Female Sprague Dawley rats were randomly divided into three groups. The rats of stem cell group were injected about 5×106Thy-1.1 stem cells into the injured artery after carotid artery injury. The rats from injury group underwent carotid artery injury and were injected with the same amount of saline; the uninjured right carotid arteries of the injury group rats were harvested for control group. The animals were killed immediately and 3, 7, 14, 21, 28 days after injury, and the samples of carotid artery were harvested. The intimal thickness was detected using histomorphological methods, and analyzed using computer image analysis. Reverse transcription-polymerase chain reaction was utilized to determine the expression of eNOS mRNA and iNOS.
RESULTS AND CONCLUSION: The intimal thickness was thinner in stem cell group compared with injury group (P < 0.05). The eNOS mRNA expression was significantly lower and iNOS mRNA was higher in injury group than in control group (P < 0.05). eNOS mRNA and iNOS mRNA expression was significantly higher in stem cell group compared with injury group (P < 0.05). These indicate that local transplantation of Thy-1.1 stem cells decreased intimal formation and could repair saccule injury, which may be associated with the over-expression of NOS and accelerated re-endothelialization following common carotid artery saccule injury.

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