Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7513-7518.doi: 10.3969/j.issn.1673-8225.2010.40.023

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Differentiation inclination of human embryonic stem cells reversibly affected by different culture systems

Luo Shu-wei, Lin Ge, Sun Zheng, Xie Ping-yuan, Lu Guang-xiu   

  1. Institute of Reproduction and Stem Cells, Central South University, Changsha  410007, Hunan Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Lu Guang-xiu, Doctoral supervisor, Professor, Institute of Reproduction and Stem Cells, Central South University, Changsha 410007, Hunan Province, China lgxdirector@yahoo.com.cn
  • About author:Luo Shu-wei☆, Doctor, Institute of Reproduction and Stem Cells, Central South University, Changsha 410007, Hunan Province, China luobosing@126.com
  • Supported by:

     the National Natural Science Foundation of China, No. 2006AA02A102 *

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Abstract:

BACKGROUND: Human embryonic stem cells (hESCs) can be maintained in feeder dependent and chemical defined culture systems, and have the capacity to differentiate into three germ layers derived cells.
OBJECTIVE: To compare the effects of feeder cells and chemical defined culture system on characteristics of hESCs.
METHODS: hESCs in feeder dependent culture system (passage 27) were transferred to chemical defined culture system for 56 passages and returned back to feeder culture system. hESCs in three culture conditions (feeder dependent culture system for 70 passages, chemical defined culture system for 56 passages or the condition hESCs transferred from chemical defined culture system to feeder dependent culture system for 13 passages and 20 passages) were analyzed by flow cytometry for SSEA4. Expression of multipotency gene and differentiation gene in three embryonic layers was determined following hESCs were induced to differentiate under three culture conditions.
RESULTS AND CONCLUSION: hESCs exhibited different differentiation inclinations in embryonic bodies conditions in feeder dependent and chemical defined culture systems, and the differentiation inclinations can be converted reciprocally. In chemical defined culture system, hESCs showed anti-differentiation to neural ectoderm cells, and this anti-differentiation can be rescued when hESCs were transferred back to feeder dependent culture system. The gene expression of Nanog may have an important role in embryonic bodies differentiation. At the same time, SSEA4 subpopulations hESCs showed the similar pattern. There may be some relationships between the SSEA4 subpopulations and differentiation inclination in feeder dependent and chemical defined culture systems.

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