Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7501-7504.doi: 10.3969/j.issn.1673-8225.2010.40.020

Previous Articles     Next Articles

Proliferation and differentiation from neural stem cells into neurons following coculture with olfactory ensheathing cells

Wang Xiu-jiao1, Sheng Bao-ying2, Huang Zuo-yi2, Zhang Xiao-mei2, Wei Chun-Jie2   

  1. 1 Department of Neurology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China; 2 Third Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi  154002 Heilongjiang Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Sheng Bao-ying, Master’s supervisor, Chief physician, Third Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi 154002 Heilongjiang Province, China SBY-N3@sohu.com
  • About author:Wang Xiu-jiao★, Studying for master’s degree, Department of Neurology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China wxj9658@sohu.com
  • Supported by:

     the Natural Science Foundation of Heilongjing Province, No.D2008-64*

PDF

406

Abstract:

BACKGROUND: External factors that effect proliferation and differentiation of neural stem cells (NSCs) contain cytokines and microenviroment. Olfactory ensheathing cells (OECs) secrete a variety of cytokines and change the microenvironment of NSCs.
OBJECTIVE: To investigate effects of different concentrations of OECs on the proliferation and differentiation of NSCs.
METHODS: NSCs and OECs isolated from Wistar rats were cultured in vitro. 5×107/L NSCs were separately cocultured with 1×107/L, 1×109/L, 1×1011/L OECs. Normal control group was set up, without OECs. The proliferation of NSCs was observed under an inverted fluorescent microscope. At 7 days after co-culture, the expression of neuron specific enolase was detected by immunocytochemistry. Ratio of positive cells to total cells was calculated.
RESULTS AND CONCLUSION: At 3 days after co-culture, three different concentrations of OECs promoted the proliferation of NSCs. The effects were significant in the NSCs + 1×109/L OECs group, compared with NSCs + 1×107/L OECs group and NSCs + 1×1011/L OECs group. At 7 days after co-culture, the percentage of neurons immunostaining cells was significantly greater in the NSCs + 1×109/L OECs group, which showed significant differences compared with normal control group (P < 0.01).

CLC Number: