Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7474-7478.doi: 10.3969/j.issn.1673-8225.2010.40.014

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Effects of bone marrow mesenchymal stem cells transplantation and growth associated protein-43 expression in brain repair

Wang Na1, Cao Wen2, Liu Guang-yi3   

  1. 1 Second Department of Spinal Column, Qingdao Orthopaedics and Traumatology Hospital, Qingdao  266021, Shandong Province, China; 2 X-ray Room, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China; 3 Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University Medical College, Qingdao  266003, Shandong Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Liu Guang-yi, Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China lgy1658@yahoo.com.cn
  • About author:Wang Na, Nurse practitioner, Second Department of Spinal Column, Qingdao Orthopaedics and Traumatology Hospital, Qingdao 266021, Shandong Province, China HAO1335686@yahoo.com.cn

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Abstract:

BACKGROUND: The bone marrow stromal cells transplantation can promote rat brain functional recovery, and have reparation function in cortex and hippocampal structure injury. These may be associated with cell self-compensation and nerve growth media, or because nervous irritability injury stimulates target tissue cells to secrete various nerve factor expression.
OBJECTIVE: To observe the role of bone marrow mesenchymal stem cells (BMSCs) transplantation on cerebral recovery and nerve regeneration after cerebral ischemia injury in rats from the angle of cell biology.
METHODS: In accordance with modified Nagasawa’s method, the middle cerebral artery occlusion/reperfusion (MCAO/R) model was established. BMSCs transplantation was performed, followed by running machine sports training and water maze test. Neurobehavioral scores, learning and memory scores were observed. TUNEL method was used to detect the expression of apoptotic neurons in cortex region and in hippocampus region, and immunohistochemical technique was utilized to determine expression change of growth associated protein-43 protein in two districts.
RESULTS AND CONCLUSION: In transplantation group, BMSCs expression was significantly increased in the cortical area and hippocampal CA1 region at 16 hours, peaked at 7 days, and the number of differentiated cells was significantly increased. Following transplantation, modified neurological severity score was lower in the transplantation group at 7, 19 and 21 days compared with model group (P < 0.01). The time of platform latency was obviously shortened in the water maze test in the transplantation group compared with model group (P < 0.05). The number of times of crossing the platform was greater in the transplantation group compared with model group (P < 0.05). The number of apoptotic cells peaked at 24 hours following ischemia/reperfusion injury. At 3 days, infarct volume was maximal. At 19 days following reperfusion, growth associated protein-43 expression peaked. These indicate that cerebral ischemia mediated neurologic impairment in rats. BMSCs transplantation contributed to neural regeneration. Up-regulation of growth associated protein-43 expression suppressed neuronal apoptosis, and further promoted the repair of cerebral infarction.

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