Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7458-7461.doi: 10.3969/j.issn.1673-8225.2010.40.011

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Intravenous transplantation of human adipose-derived mesenchymal stem cells for the treatment of spinal cord injury: Correlation factor and behavior evaluation

Qiao Xiao-jun 1,2, Yang Bo 1,2, Guan Fang-xia3, Hu Xiang 4,5, Du Ying6, Zhang Tian-xiang1, Tian Yi1, Ba Yun-tao1, Duan Xiao-bing1, Deng Xiao-hui1, Gu Chen-xi7, Lei Ning-jing3, Wang Xiao-wei8   

  1. 1 Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 2 Open Laboratory of Key Discipline of Clinical Medical Sciences, Institutions of Henan Province High Learning, Zhengzhou  450052, Henan Province, China; 3 Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 4 Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, Taizhou  225300, Jiangsu Province, China; 5 Shenzhen Beike Cell Engineering Institute, Shenzhen  518000, Guangdong Province, China; 6 Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 7 Department of Clinical Medicine, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 8 College of Life Science, Hunan Normal University, Changsha  410081, Hunan Province, China　
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Yang Bo, Master, Professor, Doctoral Supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Key Discipline of Clinical Medical Sciences, Institutions of Henan Province High Learning, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Qiao Xiao-jun★, Studying for master’s degree, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Key Discipline of Clinical Medical Sciences, Institutions of Henan Province High Learning, Zhengzhou 450052, Henan Province, China qiaoxiaojun84@163.com Guan Fang-xia, Doctor, Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com
  • Supported by:

    the Zhengzhou University ‘211 project-phase Ⅲ’*; the Public Technology Service Platform of Stem Cells and Biotherapy of Jiangsu Province, No. BM2008146*

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Abstract:

BACKGROUND: As a convenience derived stem cells, adipose-derived mesenchymal stem cells (ADMSCs) have been confirmed that it can be differentiated into neuron-like cells after induced with inducer like Danshen root in vitro. Therefore, ADMSCs have the possibility to become a new seed cell which can treat spinal cord injury.
OBJECTIVE: To explore the effects of tail vein transplantation with ADMSCs on rat behaviors of acute closed spinal cord injury and expressions of various factors in spinal cord tissue.
METHODS: Human ADMSCs were isolated and cultured in vitro under sterile conditions. When ADMSCs were transmitted into the fourth generation, the cell concentration was adjusted to 1×109/L. Model of spinal cord injury was established in rats of saline control and cell transplantation groups. At 1 week following model induction, 1 mL stem cell suspension was injected into the tail vein of rats from the cell transplantation group. The same volume of saline was injected into the saline control group. No treatment was given in the model control group.
RESULTS AND CONCLUSION: Compared with the model control group and saline control group, the motor function of the hind limb was significantly recovered in the cell transplantation, but BBB score was significantly increased (P < 0.05). Expression of glial fibrillary acidic protein was significantly decreased (P < 0.05). Positive expression of neuron specific enolase and nestin was significantly increased (P < 0.05). At 3 days and 1 week following transplantation, fluorochrome-labeled ADMSCs were visible in the damaged region and surrounding segments, mainly in 1 cm range of damaged spinal cord segment, showing uneven distribution. These indicated that after the rats which suffered from acute closed spinal cord injury were transplanted via the tail vein with ADMSCs, their behaviors were improved, and the differentiations of neuronal cells were significantly increased in local damaged regions, resulting in rapid repair.

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