Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7439-7442.doi: 10.3969/j.issn.1673-8225.2010.40.007

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Potential of human umbilical cord mesenchymal stem cells differentiating into osteoblasts

Zhala Ga-hu1, Chen Li1, Lan Xiao-xia2, Chen Xiao3, Chen Xiao-yi1   

  1. 1 Department of Cell Biology, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China; 2 Research Room of Epidemiology, Department of Preclinical Medicine, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China; 3 Department of Gynaecology and Obstetrics, Hospital Affiliated to Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Chen Xiao-yi, Master, Master’s supervisor, Professor, Department of Cell Biology, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China chenxiaoyi6111@163.com
  • About author:Zhala Ga-hu★, Master, Lecturer, Department of Cell Biology, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China Zhalaga2003@sina.com
  • Supported by:

    the Scientific Research Program of Chinese People’s Armed Police Forces, No. WY200806*

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Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (UCMSCs) were rich and more primordial, had strong differentiation ability and low immunogenicity, suitable for transplanting to different individuals. Therefore, UCMSCs were an ideal target cells for cell therapy.
OBJECTIVE: To study the isolation and culture of UCMSCs and to explore the potential of osteogenesis differentiation in vitro. 
METHODS: UCMSCs were sterilely isolated and cultured. The third passage of cells were divided into induction group and control group. The induction group was treated with ossify induction medium, and control group was treated with stem cell culture medium. The cell morphology was observed under an inverted light microscope. Cell proliferation was determined by MTT assay. The cell viability was detected by double immunofluorescent staining. Cell cycle and cell surface marker were determined using flow cytometry. Following induction, alkaline phosphatase (ALP) activity was measured utilizing microplate reader. Calcium deposition was analyzed using Von Kossa staining. mRNA expression of osteopontin (OPN), ALP and bone sialoprotein (BSP) was measured by reverse transplantation-polymerase chain reaction (RT-PCR).
RESULTS AND CONCLUSION: The passage cells were steady in the cell shapes, with good viability, and highly expressed CD44. Following induction, the cells were positive in von Kossa staining. ALP activity was higher in induction group compared with control group (P < 0.05). The ALP activity was greatest on day 21 (P < 0.05). RT-PCR indicated that ALP mRNA expression was higher in induction group compared with control group (P < 0.05). The BSP and OPN genes were expressed in induction group, which suggested that human UCMSCs can differentiate into osteoblasts.

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