Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7430-7434.doi: 10.3969/j.issn.1673-8225.2010.40.005

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Survival and growth of human bone marrow mesenchymal stem cells in different infusion solutions

Li Fang1, Dong Li-yuan2, Chen Yan3, Zhang Jian-lin1, Niu Bo 1,4　   

  1. 1 Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 2 Department of Gynecology and Obstetrics, First Affiliated Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 3 Department of Respiratory Medicine, Second Affiliated Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 4 Department of Biotechnology, Capital Institute of Pediatrics, Beijing  100020, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Niu Bo, Doctoral supervisor, Professor, Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Department of Biotechnology, Capital Institute of Pediatrics, Beijing 100020, China niubo2004@126.com
  • About author:Li Fang☆, Studying for doctorate, Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China Fangli10@hotmail.com

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) transplantation technology has developed rapidly and the number of patients requiring remote transplantation is increasing gradually. However, the study on cell preservation and transportation is currently inadequate.
OBJECTIVE: To compare the effects of common infusion solutions and the infusion solution prepared by our lab on the survival and growth of MSCs.
METHODS: Bone marrow of healthy volunteer was extracted to isolate and culture human bone marrow mesenchymal stem cells (hBMSCs) by density gradient centrifugation and adherence screening methods. Cell morphology, cell surface markers and multi-directional differentiation potential were observed and identified. The third-generation hBMSCs were preserved using 10% fetal bovine serum-Dulbecco’s modified Eagle’s medium (FBS-DMEM), the infusion solution prepared by our lab and 5 kinds of common infusion solutions (9 g/L sodium chloride injection, 50 g/L glucose injection, 50 g/L glucose and sodium chloride injection, 9 g/L sodium chloride injection containing 2% human albumin, 9 g/L sodium chloride injection containing 5% human albumin)at  25 ℃ for 1, 2, 4 and 6 hours, cell survival rate were determined. Then, the third-generation hBMSCs were preserved using the infusion solution prepared by our lab at 4 ℃ for 2, 4, 8, 12 and 24 hours. Cell survival rate and cell growth curve were determined.
RESULTS AND CONCLUSION: The third-generation hBMSCs were uniform long spindle, closely arranged parallel or swirling. hBMSCs highly expressed CD44 and CD105, and were negative for CD45. After 3 weeks of osteogenic induction, long-spindle BMSCs became polygonal, and von Kossa staining showed the typical black calcified nodules. After 2 weeks of adipogenic induction, cells became short spindle or oval, and oil red O staining showed dense red-stained lipid droplets. At 25 ℃, the survival rate of hBMSCs stored in the infusion solution prepared by our lab was much higher than hBMSCs stored in 5 kinds of common infusion solutions mentioned above. At 4 ℃, the survival and growth ability of hBMSCs stored in the infusion solution prepared by our lab and 10% FBS-DMEM were identical essentially. Results suggested that the infusion solution prepared by our lab according to cell culture medium is a more appropriate solution for maintaining cell activity and extending the extension of cell survival.

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