Abstract:

BACKGROUND: Conventional detection methods of stem cell tracking are lack of succession and noninvasion, because most of them need animal organizations to be inspected.
OBJECTIVE: To identify the effect of Feridex on human bone marrow mesenchymal stem cells (BMSCs) activities and determine suitable concentration of Feridex and screen sequence of magnetic resonance imaging (MRI) scan, through labeling BMSCs with Feridex ex vivo.
METHODS: Passage 3 BMSCs in good condition were labeled with Feridex of different concentrations (100, 50, 25, 12.5 mg/L). The culture was performed for 24-48 hours. Blank control group included Passage 3 BMSCs that were not labeled. Proliferation of BMSCs of the two groups was detected with methyl thiazolyl tetrazolium (MTT), and growth curve was made. The labeling was identified with Prussian blue staining. MRI scan of BMSCs labeled with Feridex was performed to determine the suitable sequence of MRI scan.
RESULTS AND CONCLUSION: There was no effect of Feridex with concentration ≤25 mg/L on BMSCs, and the result of labeling with concentration of 25 mg/L was optimal. Feridex with concentration≥ 50 mg/L would obviously inhibit the proliferation of BMSCs. Results of MRI scan revealed that MRI signal of BMSCs Feridex labeled for 24 and 48 hours or the filial Passage degraded in all sequence T1WI, T2WI and T2*WI3, the change of T2*WI was mostly obvious. These results indicate that Feridex can be used for ex vivo labeling of BMSCs for succession research. The effect of Feridex on BMSCs was in concentration dependablity, and concentration of 25 mg/L was optimal. The result of sequence T2*WI of MRI scan was more suitable to trace Feridex-labeled human BMSCs