Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7415-7419.doi: 10.3969/j.issn.1673-8225.2010.40.002

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Differentiation of superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells into cardiomyocytes-like cells in vitro induced by 5-azacitidine

Liu Yong-hao1, Guo Liang1, He Mi-chun2, Shen Zhen-ya2   

  1. 1 Image Center, 2 Department of Cardiovascular Surgery, First Affiliated Hospital, Soochow University, Suzhou  215006, Jiangsu Province, China　
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Guo Liang, Professor, Image Center, First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China ilguoliang@sohu.com
  • About author:Liu Yong-hao★, Master, Attending physician, Image Center, First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China lyh0902@126.com
  • Supported by:

    the Natural Science Foundation of Jiangsu Province, No. BK2006704-1*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) induced by various inducers can differentiate into cardiomyocyte-like cells in vitro. The common inducer was 5-azacitidine. Superparamagnetic iron oxide (SPIO) particle has small diameter and can be phagocytized by stem cells. Its paramagnetism can be detected by magnetic resonance imaging (MRI). Thus, SPIO has been an ideal tracer of transplanted stem cells in living bodies.
OBJECTIVE: To observe the effects of differentiation of SPIO-labeled stem cells into cardiomyocytes-like cells in vitro induced by 5-azacitidine, and compare with unlabeled stem cells.
METHODS: Pig BMSCs were isolated and cultured. In the experimental group, the third passage of BMSCs were marked with SPIO, and then 5-aza was added in vitro for 24 hours. In the control group, BMSCs were induced by 5-azacitidine directly. 2 weeks later, the induced cells were evaluated with desmin, myosin heavy chain, cardiac troponin I and connexin-43 by immunocytochemical staining. Iron particles were detected by Prussian blue staining.
RESULTS AND CONCLUSION: Desmin, myosin heavy chain, cardiac troponin I, connexin-43 were expressed in BMSCs induced by 5-azacitidine. Prussian blue staining showed numerous blue-stained iron particles in the cytoplasm of SPIO marked BMSCs. These indicate that SPIO-marked BMSCs can differentiate into cardiomyocytes-like cells under 5-azacitidine induction in vitro.

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