Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (31): 5753-5757.doi: 10.3969/j.issn.1673-8225.2010.31.014

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Protective effect of hyperoxic fluid on pancreas transplantation in rats

Liang Zhong-ping1, Lao Xue-jun2, Ding Hong-wen2, Su Ze-xuan 2,3   

  1. 1 Department of Gastrointestinal Surgery, Qingyuan People’s Hospital, Fifth Affiliated Hospital of Jinan University, Qingyuan  511500, Guangdong Province, China; 2 Transplantation Center, First Affiliated Hospital of Jinan University, Guangzhou  510632, Guangdong Province, China; 3 Graduate College of Southern Medical University, Guangzhou  510525, Guangdong Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: Su Ze-xuan, Professor, Doctoral supervisor, Transplantation Center, First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China; Graduate College of Southern Medical University, Guangzhou 510525, Guangdong Province, China suz2008@126.com
  • About author:Liang Zhong-ping☆, Doctor, Attending physician, Department of Gastrointestinal Surgery, Qingyuan People’s Hospital, Fifth Affiliated Hospital of Jinan University, Qingyuan 511500, Guangdong Province, China liangzp2006@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 04010486*; Foundation of Health Department of Guangdong Province, No. A2004337*

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Abstract:

BACKGROUND: Organ for transplantation is insufficient, and primary transplantation of nonfunction caused by perfusion cryopreservation occasionally occurs. It has clinically significant to reduce organ damage caused by perfusion preservation.
OBJECTIVE: To study the protective effect of hyperoxic perfusion fluid on pancrease transplantation in rats.
METHODS: Totally 40 Wistar rats were randomly divided two groups and poured with natrium lacticum ringer’s solution or hyperoxic natrium lacticum ringer’s solution. After perfusion, the tail pancreases of rats were checked by electron microscopy. The glucose concentrations and acute rejection score of pancreas tissues were compared between two groups at 1 and 3 days after pancreas transplantation.
RESULTS AND CONCLUSION: There was no statistics difference between two groups prior to operation. The ultrastructure of pancreatic tissue was basically normal, with completely nuclei and plenty of mitochondria in the hyperoxia liquid group after perfusion. The pancreatic tissues still orderly arranged in the control group, with obviously swelling in mitochondria, and parts of mitochondria were vacuolar degenerated. The glucose concentrations of the hyperoxia liquid group were lower than that of the control group at 1 and 3 days after transplantation (P < 0.05). The acute rejection scores of the hyperoxia liquid group were lower than that of the control group (P < 0.05). Thus, hyperoxia liquid can mitigate the ischemia reperfusion injury and protect pancreas transplantation in rats.

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