Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (31): 5758-5762.doi: 10.3969/j.issn.1673-8225.2010.31.015

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Construction of a recombinant human interleukin 10 against skin allograft rejection model

Feng Ji-hong1, Sun Wan-bang1, Luo Jun-min2, Liu Kai-shan1, Zhang Lei1, Wen Qiong-na1, Chen Fu-chao2, Wang Sheng-xiang1   

  1. 1 Zhuhai Campus of Zunyi Medical College, Zhuhai  519041, Guangdong Province, China; 2 Department of Immunology, Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: 孙万邦，教授，硕士生导师，遵义医学院珠海校区，广东省珠海市 519041 sunwb7224@sina.com
  • About author:Feng Ji-hong★, Master, Lecturer, Physician, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China jh_f@163.com
  • Supported by:

    the Key Program of Education Department of Guizhou Province, No. 2008032*

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Abstract:

BACKGROUND: Previous studies demonstrated that interleukin-10 (IL-10) has application prospect in preventing and treating allograft rejection, which may be served as a new immunosuppressant. Animal transplantation models provide platform for studying immunosuppressant. Mouse is widely used in establishing skin allograft model, which require highly transplantation technique.
OBJECTIVE: To establish a model of skin allograft rejection in rabbits and to study the effects of recombinant human IL-10 (rhIL-10) against skin allograft rejection.
METHODS: The skin flap from rabbit back was cut into three kinds of skin grafts: the full thickness flap with dermis, the middle thickness flap with dermis and the thin layer flap without dermis, which were transplanted into rabbits. The rabbits were randomly divided into the brake group and non-brake group, those in the former group was wore a self-made collar in order to restrict his head and neck. Models that drugs against rabbit allograft rejection were divided into three groups: rhIL-10 experimental group, CsA positive control group and negative control group. The mixed lymphocyte reactions were used to observe the proliferation index. The graft rejection time and the average survival time were continuously observed for 30 days.
RESULTS AND CONCLUSION: The effects of the donors and recipients mixed on proliferation of T lymphocytes showed that allogeneic major histocompability complex were different in rabbits, and rejection may be occurred when skins were transplanted each other. The middle thickness skin graft was optimal. The self-made neck-collars could fix and protect the transplanted wounds and skin grafts. The rabbit model of rhIL-10 against skin allograft rejection was established successfully, and rIL-10 has anti-agraft rejection effect.

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