Rapid establishment of rat liver transplantation model by portal vein preferential reverse anastomosis

doi:10.12307/2022.537

Abstract

Abstract: BACKGROUND: The traditional “two cuff” model of rat liver transplantation takes a long time to establish, which is difficult to grasp and adapt to the training of graduate students.
OBJECTIVE: As per the characteristics of multi-step establishment of liver transplantation model in rats, to rationally combine the time and method of each step so as to shorten the learning time and reduce the difficulty of operation, thereby rapidly establishing a rat model of liver transplantation.
METHODS: The portal vein was preferentially anastomosed using the two-cuff method when the blood flow of the inferior vena cava was unobstructed. The cuff was anastomosed reversely by placing the donor liver in the lower abdomen of rats (portal vein preferential anastomosis group). The rat liver transplantation model was established and compared with the traditional rat liver transplantation model (traditional transplantation group). The learning time curve, portal vein occlusion time, anhepatic phase time, inferior vena cava occlusion time, recipient operation time, and postoperative survival of the two groups were statistically analyzed.
RESULTS AND CONCLUSION: The learning curves of 32 cases in the traditional transplantation group and 30 cases in the portal vein preferential anastomosis group were analyzed. The learning time of portal vein preferential anastomosis group was shorter than that of traditional transplantation group. The operation time of 12 rats in the traditional transplantation group and 20 rats in the portal vein preferential anastomosis group which survived over 2 days was statistically analyzed. Parameter test analysis showed that the portal vein occlusion time in the portal vein preferential anastomosis group was slightly longer than that in the traditional transplantation group, but there was no significant difference. However, the blocking time of the suprahepatic inferior vena cava in the portal vein preferential anastomosis group was (15.0±2.2) minutes, which was significantly shorter than (21.3±2.1) minutes in the traditional transplantation group (P < 0.05), and the time of infrahepatic inferior vena cava occlusion in the portal vein preferential anastomosis group was (25.0±2.9) minutes, which was significantly shorter than that [(32.5±3.2) minutes] in the traditional transplantation group (P < 0.05). There was no significant difference in donor perfusion time, donor operation time, donor liver repair time and recipient operation time between the two groups. Rat model of liver transplantation with portal vein preferential anastomosis can shorten the learning cycle, reduce the difficulty of operation, and provide a relatively reliable method for beginners to quickly master the establishment of rat liver transplantation model, which is suitable for learning and promotion.

Key words: portal vein preferential anastomosis, liver transplantation, reverse anastomosis, learning curve, anhepatic phase

CLC Number:

Zhang Jixiang, Yan Hongxian, Xuan Juanjuan, Bai Hongtai, Wang Yaoquan, Wei Sidong, Chen Guoyong. Rapid establishment of rat liver transplantation model by portal vein preferential reverse anastomosis[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(17): 2696-2701.

Figures/Tables 4

References

[1] SUN Z, LI T, WEN H, et al.Immunological effect induced by mesenchymal stem cells in a rat liver transplantation model. Exp Ther Med. 2015;10(2):401-406.
[2] CHENG Y, LAN H, CHEN Y, et al. Protective Effect of Retrograde Reperfusion Against Hepatic Autophagy Impairment in Rat Liver Transplantation. Transplant Proc. 2021;53(1):443-449.
[3] BRUINSM BG, BERENDSEN TA, IZAMIS ML, et al. Supercooling preservation and transplantation of the rat liver. Nat Protoc. 2015;10(3):484-494.
[4] LI M, LU C, ZHU H, et al. Cenicriviroc ameliorates the severity of graft-versus-host disease through inhibition of CCR5 in a rat model of liver transplantation. Am J Transl Res. 2019;11(6):3438-3449.
[5] WESTERKAMP AC, FUJIYOSHI M, OTTRNS PJ, et al. Metformin Preconditioning Improves Hepatobiliary Function and Reduces Injury in a Rat Model of Normothermic Machine Perfusion and Orthotopic Transplantation. 2020;104(9):e271-e280.
[6] CHEN X, WANG L, DENG Y, et al. Inhibition of Autophagy Prolongs Recipient Survival Through Promoting CD8+ T Cell Apoptosis in a Rat Liver Transplantation Model. Front Immunol. 2019;10:1356.
[7] 赵英鹏,李立,陈刚.减体积肝移植在大鼠脂肪肝供肝肝移植模型中的应用[J].中国组织工程研究,2018,22(4):582-586.
[8] CHONG AS, ALEGRE ML, MILLER ML, et al. Lessons and limits of mouse models. Cold Spring Harb Perspect Med. 2013;3(12):a015495.
[9] KAMADA N, CALNE RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage.Transplantation. 1979;28(1):47-50.
[10] KAKIZAKI Y, MIYAGI S, SHIMIZU K, et al. The Effects of Short-term Subnormothermic Perfusion After Cold Preservation on Liver Grafts From Donors After Circulatory Death: An Ex Vivo Rat Model. Transplantation. 2018;102(4):e147-e154.
[11] 李蕾,李善宝,汪涛.单人直视下大鼠肝移植模型供肝获取不同灌注方式比较[J].器官移植,2020,11(3):356-361.
[12] 陈辉,姜骊,王雁.三种不同麻醉药对SD大鼠肝移植手术麻醉效果的比较和应用[J].实验动物科学,2009,26(4):57-59+62.
[13] JIA JJ, LI JH, YU H, et al. Machine perfusion for liver transplantation: A concise review of clinical trials. Hepatobiliary Pancreat Dis Int. 2018;17(5):387-391.
[14] GASSNER JMGV, NOSSER M, MOOSBURNER S, et al. Improvement of Normothermic Ex Vivo Machine Perfusion of Rat Liver Grafts by Dialysis and Kupffer Cell Inhibition With Glycine. Liver Transpl. 2019;25(2):275-287.
[15] JIA L, YU W, YU H, et al. Electroacupuncture Pretreatment Attenuates Intestinal Injury after Autogenous Orthotopic Liver Transplantation in Rats via the JAK/STAT Pathway. Oxid Med Cell Longev. 2020;2020:9187406.
[16] QU B, YU F, SHENG GN, et al. Protective Effect of a Novel Technique for Liver Transplantation in the Rat. Transplant Proc. 2018;50(1):267-273.
[17] XIONG L, WANG D, LIN S, et al. Soluble CD83 inhibits acute rejection by up regulating TGF-βand IDO secretion in rat liver transplantation. Transpl Immunol. 2021;64:101351.
[18] ZHAO Z, PAN G, TTANG C, et al.IL-34 Inhibits Acute Rejection of Rat Liver Transplantation by Inducing Kupffer Cell M2 Polarization. Transplantation. 2018;102(6):e265-e274.
[19] 骆助林,汪涛,田伏洲.大鼠无肝期耐受门静脉血流阻断的安全时限研究[J].山东医药,2012,52(43):11-13.
[20] 周京安,李宁.肠道耐受淤血-再灌注损伤的安全时限观察[J].临床与实验病理学杂志,2010,26(3):339-344.
[21] WANG J, YU S, LI J, et al.Protective role of N-acetyl-l-tryptophan against hepatic ischemia-reperfusion injury via the RIP2/caspase-1/IL-1β signaling pathway. Pharm Biol. 2019;57(1):385-391.
[22] YUAN Y, CHEN MH, HUANG J, et al.Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model. J Vis Exp. 2021; (169). doi: 10.3791/61779.
[23] LI X, WANG L, YANG X, et al.Metformin Attenuates Ischemia-reperfusion Injury of Fatty Liver in Rats Through Inhibition of the TLR4/NF-κB AxisBalkan Med J. 2020;37(4):196-202.
[24] BEJAOUI M, ZAOUALI MA, SAKLE R, et al.Olprinone protects the liver from ischemia-reperfusion injury through oxidative stress prevention and protein kinase Akt activation. Can J Physiol Pharmacol. 2018;96(3):227-231.
[25] ZAZUETA C, BUELNA-CHONTAL M, MACIAS-LOPEZ A, et al.Cytidine-5’-Diphosphocholine Protects the Liver From Ischemia/Reperfusion Injury Preserving Mitochondrial Function and Reducing Oxidative Stress. Liver Transpl. 2018;24(8):1070-1083.
[26] 唐丹,袁泉,王志超.冠状动脉支架在弯曲血管中的血流动力学分析[J].中国组织工程研究,2018,22(22):3563-3568.
[27] 刘洪涛,李永国.受体肝分步切除三袖套法大鼠原位肝移植术的改进[J].中国现代手术学杂志,2006,10(2):106-108.
[28] 何宇涛,周焘,李晓凯.大鼠肝移植中veno-lined支架技术的改良研究[J].中华器官移植杂志,2020,41(11):687-691.
[29] PECORA RA, CANEDO BF, ANDRAUS W, et al. Portal vein thrombosis in liver transplantation. Arq Bras Cir Dig. 2012;25(4):273-278.
[30] ONDA S, FURUKAWA K, SHIRAI Y, et al. New classification-oriented treatment strategy for portal vein thrombosis after hepatectomy. Ann Gastroenterol Surg. 2020;4(6):701-709.