Abstract:

BACKGROUND: The success probability of young donors for pancreatic or islet transplantation is great due to easy vascular anastomosis. However, the anatomical basis of young donors for pancreatic segmental transplantation are insufficient.
OBJECTIVE: To observe the microstructure, positive expression areas of pancreatic islet B cell glucagons and insulin, and immune positive staining area of pancreatic islet B cells in the pancreatic tails of young people, and to explore the pancreatic vascular anatomy of young cadavers.
METHODS: Totally 12 cases of young cadavers were randomly selected. Their pancreatic tails sections were treated by hematoxylin-eosin staining and observed under an optic microscope. The number and area of pancreatic islets were measured with micrometer gauge. And the immunohistochemical staining was done with pancreatic glucagons antiserum and insulin antiserum. Pancreatic tails of another 3 young cadavers were stained with uranium and lead. The pancreatic islet B cells, the morphology, the quantity and the secreting granules and the vascular situation of the young donors were observed.
RESULTS AND CONCLUSION: In the tissues of the young donors, the secreting granules in pancreatic islet B cells were abundant, organelles such as the endoplasmic reticula, the Golgi complex and the mitochondria were mature and advanced, the insulin islets were widely distributed, and the immune staining positive expression area of the insulin B cells was large. There were many and dense insulin B cells distributed at the pancreatic tails of the young donors, and there were many secreting granules in the insulin islets B cells. The blood vessels were suitable for end-to-side anastomosis. Since the secreting activities of pancreatic cells of the young donors were vigorous and the blood vessels were suitable for end-to-side anastomosis, the young donors are suitable for pancreatic segmental insulin islets transplantation.