Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 4941-4945.doi: 10.3969/j.issn.1673-8225.2010.27.001

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Directional differentiation and identification of bone marrow mesenchymal stem cells into structural cells for heart valves

Fu Jie, Jiang Ying-jiu, Jiang Rong   

  1. Department of Cardiothoracic Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Jiang Ying-jiu, Associate professor, Department of Cardiothoracic Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China jiangyinjiu@yahoo. com.cn
  • About author:Fu Jie, Master, Attending physician, Department of Cardiothoracic Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China 66610052@qq.com
  • Supported by:

    the Scientific Research Program of Health Bureau of Chongqing City*

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Abstract:

BACKGROUND: In the component of cardiac valve, not only endothelial cells exert effects, but also fibroblasts and smooth muscle cells participate in the construction of matrix of cardiac valve.

OBJECTIVE: To establish a simple method for obtaining large quantity of endothelial cells, smooth muscle cells and fibroblasts in vitro, and identify the morphology and surface markers of the obtained cells.

METHODS: Bone marrow mesenchymal stem cells (BMSCs) were isolated from rats by whole bone marrow culture method, then cultured in vitro, and proliferated. The third passage of BMSCs was treated in special induction medium for differentiation into endothelial cells, vascular smooth muscle cells and fibroblasts. The cells were detected under an inverted microscope to examine the cell morphology every day, and then the differentiated cells were measured by immunocytochemistry.

RESULTS AND CONCLUSION: The cells induced from BMSCs had the characteristics of endothelial cells, smooth muscle cells and fibroblasts. Results have indicated that BMSCs could be directionally differentiated into endothelial cells, smooth muscle cells and fibroblasts in vitro.

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