Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (23): 4252-4256.doi: 10.3969/j.issn.1673-8225.2010.23.017

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Bone marrow mesenchymal stem cells in repair of injured pancreas in rats

Chen Qiang1, Wang Qiu-jing2, Yang Dong-xu3, Zhou Yu-lai4   

  1. 1Department of Toxicology, School of Public Health, Jilin University, Changchun  130021, Jilin Province, China;
    2Norman Bethune Medical School, Jilin University, Changchun  130021, Jilin Province, China;
    32005 Grade, Department  of Preventive Medicine, School of Public Health, Jilin University, Changchun  130021, Jilin Province, China;
    4School of Pharmacy, Jilin University, Changchun  130021, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Zhou Yu-lai, Doctor, Professor, School of Pharmacy, Jilin University, Changchun 130021, China zhouylnew@yahoo. com.cn
  • About author:Chen Qiang, Master, Senior experimentalist, Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, Jilin Province, China strongch@yahoo. com.cn
  • Supported by:

    the National Foundation Project (863 Program), No. 2004AA205020*  

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Abstract:

BACKGROUND: The ischemic injury of pancreas is a common practical problem in the clinic, which is difficult to solve. The routine methods of prevention and treatment get little success. Bone marrow mesenchymal stem cells (BMSCs) are a widely accepted modus that is developed in the recent years to treat injury, so it is necessary to find an effective way to treat the ischemic injury of pancreas using stem cells.
OBJECTIVE: To study the repair effect of BMSCs on ischemic injury of pancreas.
METHODS: Adherent culture and tissue culture were used to get highly purified BMSCs, which were incubated in L-DMEM containing 20% fetal bovine serum. Following three subcultures, BMSCs were labeled by enhanced green fluorescent protein recombinant adenovirus by injection into local pancreas of animal models where ischemic injury happened. Survival rate of the rats was observed, and the position of the marked cells in frozen sections was observed by confocal microscopy.
RESULTS AND CONCLUSION: Highly purified BMSCs were harvested from adherent culture. The survival rate was significantly higher in injected animals than the control rats. The results of confocal microscopy indicated that after 24 hours of the injection, the cells reached the ischemic part and aggregated in the microcirculatory system, and then entered the injured part gradually and heavily, finally got highly expressed in the microcirculatory system and conduit system. Results have suggested that the survival rate of the rats can be heightened by local injection of BMSCs, and this is propitious to the recovery of ischemic pancreas.

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