Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (23): 4243-4246.doi: 10.3969/j.issn.1673-8225.2010.23.015

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Migration and localization of bone marrow mesenchymal stem cells labeled by DAPI in the brain of glioma rat models

Yu Yin1, Yang Hong-fa2, Shao Jia-jia3, Wu Wei2, Zhu Dong2, Jiang Tao1, Guo Yong-chuan1, Liang Qian-lei1, Li Yan-xin1   

  1. 1Norman Bethune Third Clinical Hospital, Jilin University, Changchun  130031, Jilin Province, China;
    2Norman Bethune First Clinical Hospital, Jilin University, Changchun  130021, Jilin Province, China;
    3College of Occupation Technology, Changchun  130031, Jilin Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Yang Hong-fa, Associate professor, Norman Bethune First Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China
  • About author:Yu Yin, Doctor, Norman Bethune Third Clinical Hospital, Jilin University, Changchun 130031, Jilin Province, China fooish@sina.com

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) as vector cells were transplanted in the brain for treating glioma. How to verify BMSCs are best drug carriers.

OBJECTIVE: To observe the migration and localization of BMSCs in tumor following transplantation of rat BMSCs labeled by DAPI in the brain of a rat model.

METHODS: BMSCs were cultured in vitro. Using stereotaxic apparatus, C6 cells were injected into rat brain to establish glioma models. BMSCs labeled by DAPI were injected into the rat brain using a microinjector. The rats were sacrificed at 3 and 7 days following transplantation. The localization of BMSCs in tumor and the relationship with tumor cells were observed under a confocal microscope.

RESULTS AND CONCLUSION: The glioma model in rat brain was established successfully. BMSCs labeled by DAPI can accumulate around tumor vessel actively in rat brain, and can mix together with tumor cell. These suggested that BMSCs are a good carrier of gene therapy.

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