Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (19): 3606-3610.doi: 10.3969/j.issn.1673-8225.2010.19.040

Previous Articles    

Brain-derived neurotrophic factor, ciliary neurotrophic factor and their combination for in vitro differentiation of human umbilical blood-derived mesenchymal stem cells into nerve-like cells

Huang Wei1, Miao Zong-ning1, Chen Lei2, Zhang Xue-guang2   

  1. 1Department of Critical Care Medicine, Third People's Hospital of Wuxi, Wuxi  214041, Jiangsu Province, China;
    2Department of Brain Science, General Hospital of Armed Police Forces, Tianjin   300052, China;
    3Institute of Biotechnology, Soochow University, Jiangsu Province Key Laboratory of Stem Cells, Suzhou   215007, Jiangsu Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Miao Zong-ning, Doctor, Associate professor, Department of Critical Care Medicine, Third People's Hospital of Wuxi, Wuxi 214041, Jiangsu Province, China
  • About author:Huang Wei, Master, Associate chief physician, Department of Critical Care Medicine, Third People's Hospital of Wuxi, Wuxi 214041, Jiangsu Province, China
  • Supported by:

    Traditional Chinese Medicine Bureau Planning Program of Jiangsu Province, No. HZ07095*

PDF

936

Abstract:

BACKGROUND: Umbilical cord blood mesenchymal stem cells (UCBMSCs) have been shown to differentiate into nerve-like cells under the condition of in vitro culture. Brain-derived neurotrophic factors (BDNF) combined with ciliary neurotrophic factors (CNTF) at certain concentration can in vitro induce the differentiation of UCBMSCs into high proportion of neuronal-like cells.
OBJECTIVE: To investigate the feasibility of BDNF, CNTF and their combination for in vitro differentiation of UCBMSCs into nerve-like cells.
METHODS: UCBMSCs of passage 5 were induced to differentiate into nerve-like cells using 5, 10, 20 μg/L BDNF, 5, 10, 20 μg/L CNTF or their combination. A blank control group was set, with no any interventions. Cell morphology was observed under inverted phase contrast microscope. At 1, 3, and 6 days of experiment, immunocytochemical staining for neuron specific enolase and glial fibrillary acidic protein was performed. The proportion of differentiated neuronal-like cells and glial cell-like cells was calculated.
RESULTS AND CONCLUSION: After induced differentiation into nerve-like cells, human UCBMSCs exhibited changeable morphology with contracted cell body, enhanced refraction of nucleus, and dendrite- and axon-like structure. Compared with blank control group, BDNF and CNTF could significantly enhance the differentiation proportion of UCBMSCs into nerve-like cells.         20 μg/L BDNF combined with 20 μg/L CNTF yielded highest differentiation proportion of human UCBMSCs into nerve-like cells. These findings indicate that human UCBMSCs can differentiate into nerve-like cells after in vitro induction of BDNF, CNTF or their combination

CLC Number: