Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (19): 3518-3522.doi: 10.3969/j.issn.1673-8225.2010.19.020

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Effect of icariin on the expression of transforming growth factor-beta 1 and bone morphogenetic protein-2 in the process of mesenchymal stem cells differentiation into osteoblasts

Yang Li1, Zhang Rong-hua1, Zhu Xiao-feng2, Cai Yu1, Huang Feng3   

  1. 1Staff Room of Traditional Chinese Medicine, School of Pharmacy, Jinan University, Guangzhou  510632, Guangdong Province, China;
    2Department of Traditional Chinese Medicine, First Affiliated Hospital, Jinan University, Guangzhou  510632, Guangdong Province, China;
    3Staff Room of Pharmacology, School of Pharmacy, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-05-07 Published:2010-05-07
  • About author:Yang Li, Doctor, Lecturer, Staff Room of Traditional Chinese Medicine, School of Pharmacy, Jinan University, Guangzhou 510632, Guangdong Province, China doctormonkey@126.com
  • Supported by:

    the National Nature Science Foundation of China, No. 30472274*;
    a Grant from Natural Science Foundation of Guangdong Province, No. 04105837*;
    an Accented and Lead Term from Social Development Project of Guangdong Province, No. 2004B33001004*

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Abstract:

BACKGROUND: As the main effective ingredient of epimedium, icariin (ICA) can promote the differentiation of mesenchymal stem cells (MSCs) into osteoblasts.

OBJECTIVE: To observe effects of ICA on the expression of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) in the process of mesenchymal stem cells differentiation into osteoblasts, and to explain the inducing mechanisms.  

METHODS: MSCs were isolated and purified by whole marrow adherent method. 20 μg/mL ICA was used to be the intervention concentration; according to the different induced conditions, MSCs were divided into 4 groups: blank group, classic group (induced by the classic osteoblast-induced system), ICA group (induced by 20 μg/mL ICA) and classic+ICA group (induced by the combination of classic osteoblast-induced system and 20 μg/mL ICA). ELISA was used to detect the expression of TGF-β1 and BMP-2 of each induced group in the progress of MSCs differenting into osteoblasts.

RESULTS AND CONCLUSION: The expression of TGF-β1 and BMP-2 of the classic group, ICA group and classic+ICA group were all higher than that of the blank group (P < 0.05); the expression of TGF-β1 of each group was higher at 7 days than that at 14 and 21 days (P < 0.01); however, the expression of BMP-2 of each group at 7, 14 and 21 days had no statistical difference (P > 0.05). The results revealed that upregulation of the expression of TGF-β1 and BMP-2 in the ICA-induced groups may be the mechanisms on improving the differentiation of MSCs into osteoblasts.

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