Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (19): 3499-3502.doi: 10.3969/j.issn.1673-8225.2010.19.016

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Effects of autologous bone marrow mobilization on vascular regeneration of acute ischemic myocardium

Yu Gui-ping1, Shen Zhen-ya2, Yu Yun-sheng2, Guo Shi-qiang2, Chen Yi-huan2, Hu Yan-qiu2   

  1. 1Department of Thoracic Surgery, Jiangyin People’s Hospital, Southeast University, Wuxi  214400, Jiangsu Province, China;
    2Department of Cardiovascular Surgery, First People’s Hospital, Soochow University, Suzhou  215006, Jiangsu Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Shen Zhen-ya, Chief physician, Doctoral supervisor, Department of Cardiovascular Surgery, First People’s Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China
  • About author:Yu Gui-ping, Master, Physician, Department of Thoracic Surgery, Jiangyin People’s Hospital, Southeast University, Wuxi 214400, Jiangsu Province, China xiaoyuer97103@163.com

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Abstract:

BACKGROUND: Animal trials have suggested that stem cell transplantation can replace damaged myocardial cells and establish new vessels for improving blood supply.
OBJECTIVE: To study the effect of autologous bone marrow mobilization using colony stimulating factor on cardiac function and new vessels in swines with acute myocardial infarction.
METHODS: A total of 10 healthy Taihu Meishan swines were divided into two groups following model establishment of myocardial infarction. In the control group, the swines were injected with DMEM through coronary artery at 4 weeks later. In the experimental group, the swines were infused with granulocyte colony-stimulating factor at 3 hours following myocardial infarction for 5 consecutive days. Cardiac function was examined using ultrasonic cardiograph at 8 weeks following myocardial infarction. Expression of serum vascular endothelial growth factor and transforming growth factor beta (TGF-ß) was detected at various time points. Hematoxylin-eosin staining was used to observe pathological changes in infarct region. Capillary density was determined using immunohistochemical staining for Ⅷ factor.
RESULTS AND CONCLUSION: Following mobilization, serum vascular endothelial growth factor and TGF-ß expression were significantly increased in the experimental group. Immunohistochemical staining for Ⅷ factor showed that vascular density was significantly increased. These indicated that colony stimulating factor-induced autologous bone marrow stem cell mobilization can obviously promote vascular regeneration of acute ischemic myocardium.

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