Abstract:

BACKGROUND: With deep research of osteoporosis mechanism, investigators mainly focused on bone marrow mesenchymal stem cells (BMSCs), the source of osteoblasts.
OBJECTIVE: BMSCs from osteoporosis rats were isolated and cultured in vitro. To observe biological features of BMSCs of rat models of osteoporosis, to analyze cytopathology mechanism of osteoporosis, and to provide a significant drug target for prevention and treatment of osteoporosis.
METHODS: The animal model of osteoporosis was established by ovariectomied method in 10-month-old female Sprague-Dawley rats. Sham operation control group was established. BMSCs were isolated from the rats and cultured in vitro by density-gradient centrifugation in both groups. Morphology of BMSCs was observed under a scanning electron microscope. The growth curve and adhesion rate were measured.
RESULTS AND CONCLUSION: Reproductive activity of BMSCs of osteoporosis rats was significantly decreased, and there were many differences in structural features compared with control rats. In vitro experiment results demonstrated that the decrease in reproductive activity in vitro of BMSCs of osteoporosis rats contributes to osteoporotic cytopathology.