Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (19): 3427-3430.doi: 10.3969/j.issn.1673-8225.2010.19.002

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Seed cells differentiated from rat bone marrow mesenchymal stem cells versus from great saphenous vein-derived cells for tissue-engineered ligament

Du Yao1, Li Xiao-sheng1, Zhang Na-juan2, Chen Tie-zhu1, Zeng Wen-kui1, Tan Cai-fu1   

  1. 1First Hospital Affiliated to Hunan Normal University, i.e., Hunan Provincial People’s Hospital, Changsha  410005, Hunan Province, China;
    2Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shaanxi Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Li Xiao-sheng, Doctor, Chief physician, First Hospital Affiliated to Hunan Normal University, i.e., Hunan Provincial People’s Hospital, Changsha 410005, Hunan Province, China lxsld2009@163.com
  • About author:Du Yao, Studying for master’s degree, First Hospital Affiliated to Hunan Normal University, i.e., Hunan Provincial People’s Hospital, Changsha 410005, Hunan Province, China Leo_du_19822188@sina.com

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Abstract:

BACKGROUND: Ligament injury in arthrosis is difficult to be healed, and conventional ligament substitutes have disadvantages. To treat arthrosis, a suitable seed cell is needed using tissue engineering method.
OBJECTIVE: To observe the possibility of cells differentiated from bone marrow mesenchymal stem cells (BMSCs) and from great saphenous vein of Sprague Dawley rats as seeded cells of ligament tissue engineering.
METHODS: Ligament without cells was obtained by a trypsin-deterging cell extraction process. Rat BMSCs were isolated and amplified by gradient centrifugation conjucted with adherent method in vitro, induced by fibroblast growth factor, and differentiated into fibroblasts. Great saphenous vein interstitial cells served as controls. Cell morphology, proliferation capability, collagen-synthesized capability and growth on acellular ligament were observed.
RESULTS AND CONCLUSION: Both BMSCs and venous interstitial cells grew adherently and appeared fusiform or polygonal. There were no significant differences in cell resuscitation rate and collagen-synthesized capability. Both cells could be implanted on ligament tissue and grew on its surface. There were no significant differences between venous interstitial cells and fibroblasts differentiated from BMSCs.

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