Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (10): 1843-1846.doi: 10.3969/j.issn.1673-8225.2010.10.028

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Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro  

Luo Hong-wu, Huang Xiang-jun, Liu Xun-yang, Huang Fei-zhou   

  1. Department of Hepatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha   413000, Hunan Province, China
  • Online:2010-03-05 Published:2010-03-05
  • Contact: Huang Xiang-jun, Master, Physician, Department of Hepatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 413000, Hunan Province, China hxiangj168@126. com
  • About author:Luo Hong-wu, Doctor, Associate chief physician, Master’s supervisor, Department of Hepatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 413000, Hunan Province, China luohongwuzny@sina.com

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Abstract:

BACKGROUND: Human amniotic epithelial cells (AECs) are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair. Currently, marking and tracing of human AECs remains rarely reported.
OBJECTIVE: To explore the efficiency of adeno-associated virus (AAV) vector-medicated green fluorescent protein (GFP) on in vitro cultured human AECs transfection.
METHODS: Human amnion samples were harvested and trypsinized to isolate human AECs. The AECs were transfected with AAV-GFP, and the transfection efficiency was detected.
RESULTS AND CONCLUSION: Human AECs were successfully primary cultured and passaged in vitro. AAV-GFP-transfected AECs stably and highly expressed GFP, with a transfection efficiency of 58%.

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