Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (10): 1813-1816.doi: 10.3969/j.issn.1673-8225.2010.10.021

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Effect of basic fibroblast growth factor on proliferation and differentiation of monkey bone marrow mesenchymal stem cells into neuronal precursor cells: Does the concentration affect cryptotanshinone induction?

Liu Xiao-gang1, Deng Yu-bin2, Cai Hui1   

  1. 1Department of Pathology, Chuiyangliu Hospital, Beijing  100022, China;
    2Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Online:2010-03-05 Published:2010-03-05
  • Contact: Deng Yu-bin, Doctor, Professor, Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China dengyub@mail. sysu.edu.cn
  • About author:Liu Xiao-gang, Master, Attending physician, Department of Pathology, Chuiyangliu Hospital, Beijing 100022, China chinalark@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30271327*;
    Science and Technology Planning Project of Guangdong Province, No. 2004A30201002*

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Abstract:

BACKGROUND: Basic fibroblast growth factor (bFGF) belongs to active peptide, which is an effective mitogenic factor.

OBJECTIVE: To investigate the effect of bFGF on proliferation and differentiation of monkey bone marrow mesenchymal stem cells (BMMSCs) into neuronal precursor cells.

METHODS: Monkey BMMSCs were in vitro cultured by density gradient centrifugation, and then divided into 4 groups after passaged, namely, control, bFGF with low, medium and high concentration groups. In the bFGF groups, 0, 3, 6, 10 μg/L bFGF were applied. The proliferation of BMMSCs in each group were observed. The 5th BMMSCs were cultured with serum free L-DMEM culture medium containing 20 mg/L cryptotanshinone to differentiated into neural-like cells. The expression of positive-nestin protein was detected by immunohistochemical method.

RESULTS AND CONCLUSION: Compared with the control group, proliferation rate of BMMSCs in the bFGF groups were accelerated (P < 0.05), which showed a positive correlation to the concentration of bFGF. The positive-nestin protein could be found in the low and medium concentration groups at 0.5 hours after induction, and reached a peak at 1.5 hours, which increased obviously in the low concentration group than that of the high concentration group (P < 0.05). bFGF can promote BMMSCs proliferation in vitro, enhance inducing ratio of prophase neuron-like cells at lower concentration but inhibit differentiation at high level.

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