Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (10): 1808-1812.doi: 10.3969/j.issn.1673-8225.2010.10.020

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Ca2+ signaling mediated salidrosides promotes directional differentiation of mouse bone marrow mesenchymal stem cells into nerve cells

Pei Jing-jing1, Wu Run1, Zhao Hong-bin2, Liu Xu-dong2, Hu Jun2, Bai Meng-hai2   

  1. 1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou  730070, Gansu Province, China;
    2General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou  730050, Gansu Province, China
  • Online:2010-03-05 Published:2010-03-05
  • Contact: Zhao Hong-bin, Doctor, Associate professor, Master’s supervisor, General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China zhao6703@yahoo. com.cn
  • About author:Pei Jing-jing, Studying for master’s degree, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China peijingjing0303@163.com
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 0710RJZA067*;
    the Research Foundation in Medical Science of Lanzhou Military Area Command, No. LXH-2007007*

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Abstract:

BACKGROUND: Tranditional Chinese medicine, which possesses anti-oxidation properties, can promote directional differentiation of mouse bone marrow mesenchymal stem cells (BMMSCs) into nerve cells. Salidrosides, as the effective constituent of Rhodiola, have strong anti-oxidation function.

OBJECTIVE: To investigate the molecule mechanism of salidrosides induced differentiation of mouse BMMSCs into nerve cells.

METHODS: When in vitro cultured BMMSCs reached 80% confluency, the cells were assigned into 3 groups. Cells in the control group were cultured by complete culture medium; those in the induction and positive control groups were cultured by complete culture medium adding 20 mg/L salidrosides or 0.1 mg/L nerve growth factors (NGF). The related gene and protein of nerve cells were detected using RT-PCR and Western blot method at 12 hours after culture. After that, the cells in the induction group were divided into 3 groups, the blocking agents EGTA (Ca2+ chelator), Nifedipine (L-type Ca2+ channel blocker) and LY294002 (IP3 receptor blocking pharmacon) were applied to block the cellular Ca2+ signal pathway respectively for 12 hours. RT-PCR and Western blot methods were used to study the signal transduction of the salidrosides.

RESULTS AND CONCLUSION: ①The expression of neuron specific enolase (NSE), β-Tubulin III, Nurr1 mRNA could be found in the induction and positive control groups, instead of the control group; The expression abundance of the positive control group was smaller than that of the induction group. The expression abundance of GFAP mRNA was very low in each group, but the c-fos mRNA was expressed abundantly in the induction group. ②Compared with the positive control group, the induction group could promote the NSE expression obviously, which was no expressed in the blank control group. ③The expression of NSE and Nurr1 were conspicuously down-regulated when the Ecto Ca2+ and L-type Ca2+ channel and IP3 receptor were blocked respectively. Salidrosides can induce the differentiation BMMSCs into nerve cells. Ca2+ signaling and IP3 dependent Ca2+ signaling pathway play an important role in transduction salidrosides signal in BMMSCs differentiation.

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