Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (10): 1729-1733.doi: 10.3969/j.issn.1673-8225.2010.10.004

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Biological characteristics of human umbilical cord mesenchymal stem cells following cryopreservation

Wang You-wei1, Han Zhi-bo2, Yan Shu-lin1, Mao Ai-bin1, Wang Bin1, Wang Ding2, Chen Ke2, Han Zhong-chao1,2   

  1. 1National Engineering Research Center of Cell Products, AmCell Gene Co., Ltd., TEDA, Tianjin 300457, China;
    2State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin   300020, China
  • Online:2010-03-05 Published:2010-03-05
  • Contact: Han Zhong-chao, National Engineering Research Center of Cell Products, AmCell Gene Co., Ltd., TEDA, Tianjin 300457, China; State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China
  • About author:Wang You-wei, Master, National Engineering Research Center of Cell Products, AmCell Gene Co., Ltd., TEDA, Tianjin 300457, China wangyouwei1981@hotmail.com
  • Supported by:

    Special Foundation Project for Technology Innovation of Tianjin, No. 08FDZDSH02900*

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Abstract:

BACKGROUND: An effective freezing-thawing technique is crucial for the clinical application of human umbilical cord mesenchymal stem cells (UC-MSCs).
OBJECTIVE: To investigate biological characteristics of UC-MSCs after cryopreservation.
METHODS: UC-MSCs were isolated from human umbilical cord and frozen in liquid nitrogen. The survival rate and the suppressive effect of γ-interferon (IFN-γ) of cryopreserved-thawed and fresh human UC-MSCs were compared. Furthermore, the multiple potentials and phenotype of UC-MSCs were estimated after cryopreservation.
RESULTS AND CONCLUSION: There was no significant difference between cryopreserved-thawed and fresh human UC-MSCs on the survival rate and the suppressive effect of IFN-γ of peripheral blood mononuclear cells (PBMCs). After cryopreservation, human UC-MSCs had the potential differentiation and the phenotype of mesenchymal stem cells.

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