Abstract:

BACKGROUND: In vitro experiment has shown that the survival time of conventional chemical induction-induced neuron-like cells differentiated from bone marrow mesenchymal stem cells (BMSCs) was short, which limited its further application. With regard to the possibility of extension of iodine-induced neuron-like cells, the survival time has not yet been professionally reported.

OBJECTIVE: To research the effects of the micro-element iodine on the survival time of neuron-like cells differentiated by BMSCs.

METHODS: Rat mesenchymal stem cells at passage 3 were obtained under sterile condition, and divided into groups A-F. In group A, iodine ion was not added. In groups B-F, iodine ion at mass concentrations of 2, 55, 90, 125 and 2 500 mg/L was added respectively. An additional blank control group was established, and simultaneously the cells were induced into neuron-like cells with dimethyl sulphoxide (DMSO). Cells following induction were subjected to immunohistochemistry. Survival time of neuron-like cells was observed under different mass concentrations of iodine ion.

RESULTS AND CONCLUSION: When mass concentrations of iodine ion were between 55 - 125 mg/L, the survival time of neuron-like cells prolonged to about 5 days and structures of induced cells were intact. From then on, the number of dead cells was gradually increased till approximately one week, all neuron-like cells died. When mass concentrations of iodine ion were 2 mg/L and 2 500 mg/L, cell survival time was from 12-36 hours. No significant difference was determined compared with group A. Till 2 or 3 days, all neuron-like cells died. Above-described results indicated that an appropriated concentration of iodine iron added in the common chemical induction may be benefit for the survival time of the neuron-like cells differentiated by BMSCs, but the effect may be negligible for the survival time of neuron-like cells induced when the added concentration of iodine iron is too low or too high.