Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 1134-1137.doi: 10.3969/j.issn.1673-8225.2010.06.039

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Glycosylphosphatidilinoditol-specific phospholipase    D expression in bone marrow mononuclear cells derived from acute leukemia patients

Xiao Guang-fen1, Chen Fang-ping2, Wang Guang-ping2, Fu Bin2, Xie Jun-ming2, Cheng Ying-ni2, Li Qun2, Jian Zai-fu2   

  1. 1Department of Hematology, Third Xiangya Hospital, Central South University, Changsha   410013, Hunan Province, China;
    2Department of Hematology, Xiangya Hospital, Central South University, Changsha   410008, Hunan Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Chen Fang-ping, Master, Professor, Doctoral supervisor, Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China henfp@public.cs.hn.cn
  • About author:Xiao Guang-fen, Doctor, Associate professor, Master’s supervisor, Department of Hematology, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China xgftjx2003@yahoo.com.cn

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Abstract:

BACKGROUND: The correlation of gycosylphosphatidilinoditol-specific phospholipase D (GPI-PLD) activity, mRNA expression to leukemia type, hepatosplenomegaly and/or lymphadenopathy has been rarely reported.
OBJECTIVE: To explore the correlation of GPI-PLD expression to leukemia type and hepatosplenomegaly and/or lymphadenopathy of acute myeloid leukemia (AML) patients. 
METHODS: Fresh bone marrow specimens were obtained from 43 newly diagnosed AML patients, 28 acute lymphocytic leukemia (ALL) patients, and 21 normal persons. Bone marrow mononuclear cells were harvested by density gradient centrifugation. GPI-anchored human placent alkaline phosphatase was used as substrate. GPI-PLD activity was determined bytriton-X114 phase partitioning procedure. GPI-PLD mRNA expression was detected by semi-quantitative RT-PCR. The relationship of GPI-PLD activity, mRNA expression and leukemia type, hepatosplenomegaly and/or lymphadenopathy was analyzed.
RESULTS AND CONCLUSION: Compared with control group, GPI-PLD activity and mRNA expression in bone marrow mononuclear cells were significantly higher in AML group (P < 0.01), while they were significantly lower in the ALL group (P < 0.01). Of 43 patients with AML patients, 13 patients had hepatosplenomegaly and/or lymphadenopathy. The GPI-PLD activity (%) and mRNA expression were significantly higher in AML patients without hepatosplenomegaly and lymphadenopathy than those patients with hepatosplenomegaly and/or lymphadenopathy (P < 0.05). These results demonstrated that GPI-PLD activity alteration is consistent with GPI-PLD mRNA expression in AML patients, and the expression levels correlate to leukemia type and hepatosplenomegaly and/or lymphadenopathy of AML patients.

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