Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 1130-1133.doi: 10.3969/j.issn.1673-8225.2010.06.038

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Expression of vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 in rat bone marrow mesenchymal stem cells

Xu Yun1, Hu Yan-lai2, Zhang Zhao-lin2, Liang Yan-hong2, Tian Hua2, Tian Guang-ping2   

  1. 1Department of Anatomy, Jining Medical College, Jining  272013, Shandong Province, China;
    2Department of Anatomy, School of Medicine, Shandong University, Jinan  250012, Shandong Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Hu Yan-lai, Master, Lecturer, Department of Anatomy, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China huyanlai@sdu.edu.cn
  • About author:Xu Yun, Master, Teaching assistant, Department of Anatomy, Jining Medical College, Jining 272013, Shandong Province, China wchxy1716@163.com.

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Abstract:

BACKGROUND: Following bone marrow mesenchymal stem cells (BMMSCs) infusion therapy, which factor promotes BMMSCs migrated to correct position is a key point, currently, adhesion molecule is thought to be playing an important role in mediating BMMSCs migration.
OBJECTIVE: To investigate the expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) in rat BMMSCs.
METHODS: BMMSCs were in vitro separated from rat bone marrow by directly adherence method. The expression of VCAM-1 and ICAM-1 were identified by using immunocytochemical staining, and the expression rates of antigen were tested by flow cytometry, in addition, their mRNA expressions were measured by RT-PCR.
RESULTS AND CONCLUSION: Immunocytochemistry demonstrated that BMMSCs weakly expressed VCAM-1, but strong expressed ICAM-1. Flow cytometry showed that the expression rate of VCAM-1 was 6%, and the expression rate of ICAM-1 was 100%. RT-PCR showed that BMMSCs expressed a low level of VCAM-1 mRNA but a high level of ICAM-1 mRNA. It revealed   under physiological condition, BMMSCs expressed a low level of VCAM-1, whereas they expressed a high level of ICAM-1.

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