Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 1068-1071.doi: 10.3969/j.issn.1673-8225.2010.06.024

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Best time for mobilization and collection of peripheral blood stem cell from healthy donors

Chen Xiao-xia, Wang Zhi-ming, Luo Xian-sheng, Xu Dan-dan, Li Xing, Lei Mei-qing   

  1. Department of Hematology, Haikou People’s Hospital, Xiangya Medical College, Central South University, Haikou   570208, Hainan Province, China
  • Online:2010-02-05 Published:2010-02-05
  • About author:Chen Xiao-xia, Associate chief physician, Department of Hematology, Haikou People’s Hospital, Xiangya Medical College, Central South University, Haikou 570208, Hainan Province, China chenxia_62@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Hainan Province, No.80571*

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Abstract:

BACKGROUND: Effective mobilization and collection of hemopoietic stem cells are initial factors for peripheral stem cell transplantation, while they make sure a permanent reconstruction of hemopoiesis. Mobilization and collection have been developed; however, the collection is more, and the yield of hemopoietic stem cells is various.

OBJECTIVE: To investigate the best time of mobilization and collection of peripheral blood stem cells from healthy donors.

METHODS: A total of 16 donors who were selected from Haikou People’s Hospital between January 2003 and December 2008 were randomly divided mobilization group (A, n=6) and mobilization + collection group (B, n=10). A group was subcutaneously injected with 5.0-10.0 μg/(kg·d) recombinant human granulocyte colony-stimulating factor (rhG-CSF) (Filgrastim), and B group was treated with rhG-CSF and intravenously injected with 10 mg dexamethasone. Peripheral stem cells were collected twice in each group. The two groups were collected at the fourth and 5th day of mobilization after the second or 4th hour subcutaneous injection of rhG-CSF. The collection was 4.0-5.0 mL, the manhandled volume was 3.0-5.0 mL, and the total blood volume was 6.7-10.1 L.

RESULTS AND CONCLUSION: Number of mononuclear cells was (4.0-8.0)×108 kg-1 in the two groups. The cell concentration of fourth hour was higher than the second hour after rhG-CSF treatment (P < 0.05). The mononuclear cell concentration of fourth hour was higher than the second hour after the fourth and 5th day of rhG-CSF treatment. Our research showed that we could collect sufficient amount of cells (to a high concentration) by one time, which had reasonable collection time – value-effectiveness relationship, when the cycle blood volume was 1.8-2.2 times of circulating blood volume.

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