Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 1019-1022.doi: 10.3969/j.issn.1673-8225.2010.06.014

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Differentiation of bone marrow mesenchymal stem cells into the cells of skin appendages in diabetic wound

Zhong Xiao-hong, Wang Ming-gang, Zhao Li-ping, Gao Xin-yu   

  1. Department of Plastic Surgery, Provincial Hospital of Anhui Medical University, Hefei   230001, Anhui Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Wang Ming-gang, Professor, Chief physician, Department of Plastic Surgery, Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China 230001 mgwang1105@sina.com
  • About author:Zhong Xiao-hong, Studying for master’s degree, Physician, Department of Plastic Surgery, Provincial Hospital of Anhui Medical University, Hefei 230001, Anhui Province, China successfuldoctor@sina.com
  • Supported by:

    the First Major Scientific Research Program of Anhui Province, No. 07021021*;
    the Natural Science Foundation of Anhui High Institutes, No. KJ2008B297*;
    the Medical Scientific Research Program of Anhui Health Department, No. 09A028*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) have multi-differentiation potential. In the acute wound, MSCs have been demonstrated to have the potential for differentiating into skin cells. However, there are few reports regarding its differentiation in diabetic wound.

OBJECTIVE: To observe the feasibility of differentiation of MSCs into the cells of skin appendages under the microenvironment of diabetic wound.

METHODS: MSCs were isolated from the bone marrow of rats, purified and cultured. Third or fourth passage MSCs were selected and labeled with 5-bromodeoxyuridine (5-BrdU). The rats were injected intraperitoneally with single administration of streptozocin to establish diabetes model. After 2 weeks, a round skin wound was made on the dorsal back of rats. BrdU-labeled MSCs at a density of 1×109/L were injected into the wound of the rats. The specimens were harvested from the wound tissues to prepare sections at 2 and 3 weeks after transplantation, followed by immunohistochemical staining with BrdU or keratin.

RESULTS AND CONCLUSION: BrdU positive cells aggregated in the epidermis, dermis and hypodermia. Some positive cells appeared in the sebaceous glands and sebaceous duct cells and expressed keratin simultaneously in serial sections. During diabetic wound healing, MSCs have the potential to differentiate into the cells of the skin appendages.

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