Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 996-1001.doi: 10.3969/j.issn.1673-8225.2010.06.009

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Human umbilical cord mesenchymal stem cells under atomic force microscope: Correlation between biological characteristics and ultrastructure

Chen Li1, Wu Ben-qing1, Zhu Hua-min2   

  1. 1Department of Newborn, Shenzhen People’s Hospital,  Second Clinical Medical College of Jinan University, Shenzhen   518020, Guangdong Province, China;
    2Medical College, Jinan University, Guangzhou   510632, Guangdong Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Wu Ben-qing, Master, Chief physician, Professor, Department of Newborn, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, China wubenqing783@126.com
  • About author:Chen Li★, Studying for master’s degree, Attending physician, Department of Newborn, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, China 5823283@qq.com
  • Supported by:

    the Key Medical Science Foundation of Shenzhen City in 2009, No. 20091998A03*

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Abstract:

BACKGROUND: The relationship between cellular morphosis and function is undetachable, but there is little investigation about ultrastructure of human umbilical cord mesenchymal stem cells (hUCMSCs).

OBJECTIVE: To study the relationship between the functions of hUCMSCs and their ultrastructure obtained by atomic force microscope (AFM).

METHODS: hUCMSCs were isolated, cultured, expanded after enzyme digestion. P3 cells were observed under AFM. Immunophenotype and cell cycle were analyzed by flow cytometry, as well as induction of the adipogenic, osteogenic differentiation of hUCMSCs were identified using Oil red O staining and alkaline phosphatase staining.

RESULTS AND CONCLUSION: hUCMSCs at passage 3 were strongly positive for CD44 and CD29, weakly positive for CD106, but negative for hematopoietic marker CD34. Cells in G0/G1 phase accounted for 80%. Proliferation index was 19.9%. Following adipogenic induction, alkaline phosphatase staining demonstrated brown cytoplasm in cube and polygonal cells. AFM showed hUCMSCs were spindle shape, obvious cytoskeletal filament that connected into nets, which fit for the strong capacities for proliferation, migration and differentiation.

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