Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 992-995.doi: 10.3969/j.issn.1673-8225.2010.06.008

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Effects of folic acid on pERK1/2 expression of neural stem cells from neonatal rats under hypoxia condition in vitro

Yang Yang, Huang Guo-wei, Zhang Xu-mei, Zhao Lin, You Qing-ju, Liu Jia-jie   

  1. College of Public Health, Tianjin Medical University, Tianjin   300070, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Huang Guo-wei, Doctor, Professor, Doctoral supervisor, College of Public Health, Tianjin Medical University, Tianjin 300070, China guoweihuang@yahoo.com.cn
  • About author:Yang Yang, Studying for master’s degree, College of Public Health, Tianjin Medical University, Tianjin 300070, China yueyangzhen@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No.30771797*;
    the Science Foundation of Tianjin Medical University, No.2008Ky12*

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Abstract:

BACKGROUND: Previous studies have verified that under normal culture of neural stem cells (NSCs), folic acid can accelerate proliferation of NSCs by phosphorylation of mitogen activated protein kinase path activation ERK1/2.

OBJECTIVE: To investigate the effect of folic acid on NSC extracellular signal regulatory protein kinase pERK1/2 under hypoxic condition.

METHODS: NSCs from Neonatal rats were cultured in vitro by serum-free culture method, and incubated in a flask at 1×108/L. Except normal control group, self-made hypoxia equipment was used in the hypoxia model, folic acid deficiency and folic acid supplemented groups at day 3. At 37 ℃, hypoxia culture was conducted in the thermostat for 6 hours. The contents of folic acid were 4 mg/L, 4 mg/L, 0.65 mg/L, 8 mg/L in the four groups. Cells following 6 days were collected to count the density using trypan blue. RT-PCR was utilized to detect pERK1/2 mRNA expression. Western blot assay was employed to determine pERK1/2 protein expression.

RESULTS AND CONCLUSION: Compared with the normal control group, the proliferation of NSCs and the expression of ERK1/2mRNA and pERK1/2 protein were decreased significantly in the hypoxia model group. Compared with the hypoxia model group, the proliferation of NSCs and the expression of ERK1/2mRNA and pERK1/2 protein were increased in the folic acid supplemented group, whereas decreased in the folic acid deficiency group. There were significant differences among groups (P < 0.001). Above-described results verified that folic acid supplementation can activate ERK1/2 phosphorylatin and accelerate proliferation of NSCs under hypoxia condition.

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