Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 985-991.doi: 10.3969/j.issn.1673-8225.2010.06.007

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Survival and migration of transplanted neural stem cells: Can it elevate the efficiency of transplantation by cerebellar fastigial nucleus stimulation?

Huang Zuo-yi1, Wu Cheng-ji1, Zhu Xiao-feng2, Dong Shu-xin1, Wei Chun-jie1   

  1. 1Third Department of Neurology, First Affiliated Hospital of Jiamusi University, Jiamusi  154002, Heilongjiang Province, China;
    2Institute of Neuroscience, Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Zhu Xiao-feng, Professor, Institute of Neuroscience, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • About author:Huang Zuo-yi, Master, Professor, Third Department of Neurology, First Affiliated Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China wuchengji12@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30540062*;
    Natural Science Foundation of Heilongjiang Province, No. D2006-17*

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Abstract:

BACKGROUND: The discrepancy of neural stem cells (NSCs) transplantation is low cell survival rate and poor differentiation, which can not relieve the large apoptosis of neuron and endothelial cells in ischemic penumbra. This paper proposes a principle of overall intervention to provide an optimal environment for transplanted cells. 

OBJECTIVE: To observe the effect of cerebellar fastigial nucleus electrical stimulation (FNS) on survival and migration of NSCs.

METHODS: The EGF-responsive hippocampal NSCs of neonate Wistar rats were isolated and cultured in vitro. They were labeled by Brdu and induced by embryo cattles blood serum. The multi-differentiation potential was studied. Totally 80 rats were assigned into 4 groups. FNS+NSCs transplantation (n=32): at 24 hours FNS, right middle cerebral artery occlusion (MCAO) was prepared and received NSCs transplantation. FNS group (n =8): received the same procedure as FNS+NSCs transplantation except substitute PBS for NSCs. NSCs transplantation group (n=32): concentric electrode was inserted without electrify, the remained procedure was the same to FNS+NSCs transplantation group. Control group (n=8): concentric electrode was inserted without electrify, the remained procedure was the same to FNS group. The neural functions were recorded at hours 6, 24 and days 3, 7, 14, 28 after infarction. Rats were sacrificed at days 3, 7, 14 and 28 after transplantation, and the survival and migration of NSCs were investigated by Brdu immunocytochemical staining.

RESULTS AND CONCLUSION: Isolated and purified epidermal growth factor-responsive hippocampal NSCs were Nestin-positive and had ability of self-renewing and multi-directional differentiation. More than 85% NSCs expressed the antigen of Brdu after Brdu labeled. The functional scores of the FNS+NSCs transplantation group were significant better than those of the other 3 groups at 28 days after transplantation (P < 0.05-0.01). The number of survival cells in the FNS+NSCs transplantation group was significantly greater than that of NSCs transplantation group at days 14 and 28 after transplantation. FNS can improve the survival rate of transplanted NSCs and the functional scores following MCAO. The grading-up global environment can improve the substitution role of transplanted NSCs at local cerebral infarction damage.

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