Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (6): 967-972.doi: 10.3969/j.issn.1673-8225.2010.06.004

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Differentiation of bone marrow stromal cells into neuron-like cells induced by brain tissue extract from ischemia/reperfusion rats in vitro

Han Wei1, Gong De-zheng2, Li Xiao-mei1, Lu Qiong1, Yin Li1, Wu Wei-hua1   

  1. 1Department of Basic Medicine, Huaihua Medical College, Huaihua   418000, Hunan Province, China;
    2College of Basic Medicine, Dalian Medical University, Dalian   116044, Liaoning Province, China
  • Online:2010-02-05 Published:2010-02-05
  • About author:Han Wei, Master, Associate professor, Department of Basic Medicine, Huaihua Medical College, Huaihua 418000, Hunan Province, China yihw1966@163.com
  • Supported by:

    the Foundation Program of Department of Education of Hunan Province, No.08C042*

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Abstract:

BACKGROUND: Differentiation of bone marrow stromal cells (BMSCs) into neurons requires two processes: orientation and differentiation. Orientation and differentiation are results from different gene expression in cells with the same gene bank. Gene expression requires a certain condition. Changes in extracellular matrix can induce changes in cell morphology and gene expression manner.

OBJECTIVE: To explore the possibility of BMSC differentiation into neuron-like cells under tissue extract from rat damaged brain.

METHODS: The fifth passage of green fluorescent protein (GFP)-transfected BMSCs was induced to differentiate in brain tissue extract from ischemia/reperfusion rats or normal rats. A blank control was set. Cell morphology change was observed under the phase contrast microscope, and then evaluated using immunohistochemical staining.

RESULTS AND CONCLUSION: Primarily cultured BMSCs were purified and amplified, and then showed even spindle shape. The third passage of BMSCs was positive for CD44 and CD106, but negative for CD34. Under the fluorescence microscope, BMSCs showed fluorescence expression, but the strength was weak 24 hours following GFP transfection. Numerous cells presented significant green fluorescence 48 hours later. Following adding brain tissue extract from ischemia/reperfusion rats. Induced cells presented neuron-like feature, but neuron specific enolase specific antibody presented positive expression. Compared with the blank control group, the differentiation rate of BMSCs was significantly increased in the ischemia/reperfusion group and normal group (P < 0.05). The increased range was significantly greater in the ischemia/reperfusion group than the normal group (P < 0.05). These results indicated that brain tissue extract from ischemia/reperfusion rats can successfully induce the differentiation of BMSCs into neuron-like cells.

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