Chinese Journal of Tissue Engineering Research ›› 2026, Vol. 30 ›› Issue (1): 43-49.doi: 10.12307/2025.927

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Effect of miR-196b-5p in adipose-derived stem cell exosomes on burn wound healing in rats

Zuo Na, Tang Qi, Yu Meng, Tao Kai   

  1. Department of Burn Plastic Microsurgery, Fourth Affiliated Hospital of China Medical University, Shenyang 110000, Liaoning Province, China
  • Received:2024-10-11 Accepted:2024-12-03 Online:2026-01-08 Published:2025-06-16
  • Contact: Tao Kai, MD, Chief physician, Department of Burn Plastic Microsurgery, Fourth Affiliated Hospital of China Medical University, Shenyang 110000, Liaoning Province, China
  • About author:Zuo Na, MS, Attending physician, Department of Burn Plastic Microsurgery, Fourth Affiliated Hospital of China Medical University, Shenyang 110000, Liaoning Province, China
  • Supported by:
    Liaoning Provincial Science and Technology Plan, No. 2021JH2/10300108 (to TK)

Abstract: BACKGROUND: Currently, miR-196b-5p has been found to play a role in cell proliferation, migration and inhibition of scar hyperplasia, but there is a lack of relevant studies on whether it plays a role in the process of wound healing.
OBJECTIVE: To investigate the effect of miR-196b-5p in adipose stem cell-derived exosomes on burn wound healing. 
METHODS: The models of deep second degree skin burn in SD rats were established and randomly divided into four groups: blank control group, exosome group, agomiR-196b-5p group, and exosome + antagomiR-196b-5p group, with 10 rats in each group. PBS, adipose-derived stem cell-derived exosomes, miR-196b-5p agonist, and miR-196b-5p inhibitor were injected around the wound according to different groups. Wound healing was observed immediately after injury and on days 7, 14, and 21 after injury. On day 7 after surgery, hematoxylin-eosin staining was used to observe the expression of inflammation in the wound surface. On day 14 after suregery, Masson staining was used to observe the expression of collagen and immunohistochemical staining was used to observe the expression of CD31 in the wound surface. On day 7 after surgery, western blot assay was performed to detect the expression of α-smooth muscle actin and type I collagen in the wound surface. 
RESULTS AND CONCLUSION: (1) The wound healing was faster in the agomiR-196b-5p group, while it was slower in the blank control group and the exosome+antagomiR-196b-5p group. (2) Compared with the blank control group and the exosome+antagomiR-196b-5p group, the wound infiltration of inflammatory cells was less in the exosome group and the agomiR-196b-5p group, and the expression of CD31 was significantly increased (P < 0.01). (3) Compared with the blank control group and the exosome+antagomiR-196b-5p group, the expression levels of α-smooth muscle actin and type I collagen were increased in the exosome group and the agomiR-196b-5p group (P < 0.05). These findings indicate that miR-196b-5p in adipose stem cell-derived exosomes can promote burn wound healing in rats.

Key words: adipose-derived stem cell, exosome, miR-196b-5p, burn, wound healing, inflammatory response, angiogenesis, engineered exosome

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