Effect of iron overload on cartilaginous tissue in a mouse model of knee osteoarthritis evaluated by micro-CT

doi:10.12307/2023.651

Abstract

Abstract: BACKGROUND: Knee osteoarthritis may be related to abnormal iron metabolism, and it is very meaningful to understand the effect of iron overload on knee osteoarthritis.
OBJECTIVE: To explore the effect of iron overload on the incidence of knee osteoarthritis.
METHODS: Thirty 7-week-old C57B/6J wild-type mice were selected and randomly divided into a normal control group, an operation control group, and an operation+iron dextran group, with 10 mice in each group. At the age of 8 weeks, the operation+iron dextran group received intraperitoneal injection of iron dextran 500 mg/kg, once a week, and the injection was terminated at the age of 18 weeks. At the age of 10 weeks, the operation control group and the operation+iron dextran group received left posterior knee medial meniscus instability surgery. All mice were sacrificed at 18 weeks of age. Tibial microstructure parameters were detected by micro-CT. Safranin O-fast green staining was used to observe the degree of pathological damage of articular cartilage. Prussian blue staining confirmed the deposition of iron in mouse joints.
RESULTS AND CONCLUSION: (1) Micro-CT test showed that compared with the normal control group, the relative trabecular volume, trabecular thickness and trabecular number of subchondral bone were significantly decreased (P < 0.05), and the trabecular separation and structural model index were increased (P < 0.05) in the operation control group and operation+iron dextran group. Compared with the operation+iron dextran group, relative trabecular volume, trabecular thickness and trabecular number were increased (P < 0.05); trabecular separation and structural model index were decreased (P < 0.05) in the operation control group. (2) Safranine O staining showed that compared with the normal control group and the operation control group, the subchondral bone of the operation+iron dextran group had serious defects and ruptures on the surface of cartilage, and the number of surface chondrocytes decreased significantly. The higher Osteoarthritis Research Society International score indicated the higher severity of arthritis. (3) Prussian blue staining results showed that a large number of iron deposition particles dyed dark blue could be seen in the synovium of the operation+iron dextran group, while there were no obvious iron deposition particles in the normal control group and the operation control group. (4) These results demonstrate that iron overload can aggravate the damage of knee osteoarthritis, and increase the loss of cartilage proteoglycan components and the bone loss of subchondral bone, clarifying the relationship between iron overload and knee osteoarthritis.

Key words: knee osteoarthritis, iron overload, micro-CT, knee, Safranine O staining, Prussian blue staining, subchondral bone, OARSI score

CLC Number:

Li Shaocong, He Qi, Pan Zhaofeng, Yang Junzheng, Chen Bohao, Wang Haibin, Zhou Chi. Effect of iron overload on cartilaginous tissue in a mouse model of knee osteoarthritis evaluated by micro-CT[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(29): 4658-4663.

Figures/Tables 5

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