Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (31): 4975-4981.doi: 10.12307/2022.709

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Effect of eriodictyol on the imbalance of mitochondrial dynamics and apoptosis in SH-SY5Y cells induced by hydrogen peroxide

Li Suyao1, 2, Guo Minfang2, Yu Jingwen2, Meng Tao2, Mu Bingtao2, Li Mengdi1, 2, Li Na1, 2, Song Lijuan1, 3, Ma Cungen1, 2, Yu Jiezhong1, 2, 4   

  1. 1Research Center of Neurobiology, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China; 2Institute of Brain Science, Shanxi Datong University, Datong 037009, Shanxi Province, China; 3Department of Neurology, First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; 4Datong Fourth People’s Hospital, Datong 037009, Shanxi Province, China
  • Received:2021-08-24 Accepted:2021-10-11 Online:2022-11-08 Published:2022-04-25
  • Contact: Yu Jiezhong, MD, Professor, Doctoral supervisor, Research Center of Neurobiology, The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China; Institute of Brain Science, Shanxi Datong University, Datong 037009, Shanxi Province, China; Datong Fourth People’s Hospital, Datong 037009, Shanxi Province, China Ma Cungen, MD, Professor, Doctoral supervisor, Research Center of Neurobiology, The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China; Institute of Brain Science, Shanxi Datong University, Datong 037009, Shanxi Province, China
  • About author:Li Suyao, Master candidate, Research Center of Neurobiology, The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China; Institute of Brain Science, Shanxi Datong University, Datong 037009, Shanxi Province, China Guo Minfang, Master, Associate professor, Institute of Brain Science, Shanxi Datong University, Datong 037009, Shanxi Province, China Li Suyao and Guo Minfang contributed equally to this article.
  • Supported by:
    General Program of the National Natural Science Foundation of China, No. 81473577 (to MCG); Open Project of the State Key Laboratory of Molecular Developmental Biology, Chinese Academy of Sciences, No. 2020-MDB-KF-09 (to SLJ); Shanxi Provincial Applied Basic Research Project, No. 201901D211538 (to SLJ); Shanxi Provincial Colleges and Universities Science and Technology Innovation Project, No. 2019L0734 (to SLJ); Shanxi Provincial Colleges and Universities Science and Technology Innovation Project, No. 2020L0484 (to GMF); the Leading Team of Medical Science and Technology of Shanxi Provincial Health Commission, No. 2020TD05 (to MCG); Young Scientist Training Program of Shanxi University of Chinese Medicine, No. 2021-PY-QN-09 (to SLJ)

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Abstract: BACKGROUND: Abnormal mitochondrial dynamics is closely related to oxidative stress after affecting neurodegenerative diseases. Our previous studies have shown that eriodictyol can reduce nerve injury in Alzheimer’s disease, but whether it has a regulatory effect on mitochondrial dynamics is not clear.  
OBJECTIVE: To explore the mechanism of eriodictyol on SH-SY5Y cell apoptosis induced by hydrogen peroxide (H2O2).
METHODS:  SH-SY5Y cells were divided into three groups: PBS control group, H2O2 (250 μmol/L) group, and H2O2 (250 μmol/L) plus eriodictyol (10 μmol/L) treatment group (eriodictyol group). The intervention was performed for 24 hours. The kits were utilized to observe the level of malondialdehyde and the activity of superoxide dismutase. Cell morphology was observed under the microscope. TUNEL staining was used to observe the apoptosis. JC-1 staining was used to observe mitochondrial membrane potential. The expression levels of apoptotic proteins and mitochondrial fusion and fission protein-related proteins were detected by immunofluorescence staining and western blot assay.  
RESULTS AND CONCLUSION: (1) A significant decrease of malondialdehyde levels and a significant increase of superoxide dismutase activity were found in eriodictyol treated group, when compared with the H2O2 group. (2) Compared with the PBS control group, the H2O2 group showed decreased cell density, cytosolic hypertrophy, increased apoptosis, decreased mitochondrial membrane potential, decreased Bcl-2 expression, and increased Bax expression. Compared with the H2O2 group, above indexes were significantly improved in the eriodictyol group. (3) Compared with PBS control group, the expression levels of mitochondrial fission-related proteins dynamin-related protein 1 (p-DRP1) and mitochondrial fission protein 1 (FIS1) were elevated and the expression levels of mitochondrial fusion-related proteins optic atrophic protein 1 (OPA1) and mitofusin 2 (Mfn2) were decreased in the H2O2 group. Compared with the H2O2 group, eriodictyol treatment decreased the expression levels of p-DRP1 and FIS1 and increased the expression of OPA1 and Mfn2. (4) The results indicate that eriodictyol can inhibit H2O2-induced apoptosis in SH-SY5Y cells by regulating mitochondrial dynamics.

Key words: SH-SY5Y cells, Alzheimer’s disease, eriodictyol, H2O2, oxidative stress, apoptosis, mitochondrial dynamics

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