Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (18): 2874-2880.doi: 10.12307/2022.697
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Li Yufan1, 2, Lin Mingyue1, 2, Wang Chenxin1, 2, Zhang Rui1, 2, Li Yubao1, 2, Chen Li1, Zou Qin1, 2
Received:
2021-08-27
Accepted:
2021-09-30
Online:
2022-06-28
Published:
2022-01-30
Contact:
Chen Li, PhD, Experimentalist, Analysis and Testing Center, Sichuan University, Chengdu 610064, Sichuan Province, China
Zou Qin, PhD, Associate researcher, Analysis and Testing Center, Sichuan University, Chengdu 610064, Sichuan Province, China; Nano-biomaterials Research Center, Sichuan University, Chengdu 610064, Sichuan Province, China
About author:
Li Yufan, Master candidate, Analysis and Testing Center, Nano-biomaterials Research Center, Sichuan University, Chengdu 610064, Sichuan Province, China
Supported by:
CLC Number:
Li Yufan, Lin Mingyue, Wang Chenxin, Zhang Rui, Li Yubao, Chen Li, Zou Qin. Application of micro-CT in rabbit tibial osteomyelitis modeling[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(18): 2874-2880.
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建模组实验动物出现膝下局部肿胀,日活动频率显著降低,步姿偏跛。造模术后2周,膝下局部脓肿,见图3A,取样可见有骨膜反应,骨组织未见明显肿胀,见图3F。术后4周,膝下脓肿隆起明显,见图3B,取样后可见造模处周围有黄色黏稠脓性液体及粒状脓性组织,见图3C,骨肿胀明显并出现炎性反应,见图3G。术后6周,膝下皮肤溃烂,致病菌已从骨组织内流出,导致周围软组织较重的感染,窦道形成同时有渗出物自窦道流出,流出物色黄,稍黏稠,内部组织暴露,见图3D,大面积骨质增生腓骨与胫骨出现粘连迹象,见图3H。对照组胫骨骨面光滑,无肿胀隆起,见图3E。 2.3 X射线影像学观察结果 由X射线片可见,对照组骨连续性好,骨皮质光滑完整,无骨破坏及骨膜反应,见图4A。建模组术后2周,摄胫骨侧位片,可见轻微骨膜反应,骨破坏不明显,见图4B。术后4周,胫骨骨膜反应与骨破坏明显,病变范围向骨窗周围扩张,见图4C。术后6周,胫骨明显肿胀,骨膜反应加剧,骨密度降低,骨皮质变薄,骨溶解,骨干畸形和疑似软组织炎性阴影,见图4D。实验兔Laurence评分显示,2周最高 5 分,最低 2 分,平均(3.60±0.88)分;4周最高10分,最低5分,平均(6.80±1.36)分;6周最高11分,最低6分,平均(7.60±1.52)分,见表2。平均值渐升说明炎症随时间逐渐加剧,而标准偏差增加说明不同动物对炎症的耐受程度不同,受个体差异影响较大。"
2.4 Micro-CT分析结果 2.4.1 Micro-CT影像分析结果 由Micro-CT断层影像可见,对照组胫骨影像解剖结构清晰可见,对比度和锐利度良好,未出现明显伪影和噪声,骨髁松质骨均匀,骨小梁结构连续,厚度分布均匀,呈现规则的海绵网状结构,见图5A1,皮质骨形貌完整,无异常的肿胀或缺损,见图5A2-4。建模2周,骨髁松质骨骨质增生,骨小梁结构连续性改变,少量骨小梁断裂,见图5B1,皮质骨出现空洞,见图5B2-4,骨质破坏,骨窗及骨窗上部骨质疏松明显。建模4周,骨髁松质骨部分骨小梁变细,断裂,大部分骨小梁的连接消失,见图5C1,骨窗及上下层皮质骨对比对照组发生明显肿胀,见图5C2-4。建模6周,骨髁松质骨部分骨小梁断裂破碎,连续性破坏,结构较为散乱,见图5D1。皮质骨变薄有缺损空洞形成,骨外面有广泛性的层状高密度影,骨膜反应明显,见图5D2-4。"
2.4.2 三维重建结果 对实验兔胫骨进行三维重建可见,对照组骨面光滑,无隆起或增生,无死骨形成,骨松质与骨密质可清晰区分,内部可见对照组骨内膜完整光滑,骨髁松质骨均匀,骨小梁结构清晰,无骨质破坏,见图6A。由2周后的重建图像可见,造模处周围有轻微骨膜反应,见图6B1→,肿胀不明显,内部无明显病变,骨髁处松质骨有少量破损断裂,见图6B2。建模4周,骨窗周围可见明显的骨质增生,见图6C1○,肿胀明显;内部图像显示松质骨结构连续性破坏,大量骨小梁断裂破损,向骨窗处增生,见图6C2→,骨膜有炎症反应,见图6C2○。建模6周,骨窗周围有大量死骨形成且骨膜反应剧烈,整体明显肿胀,骨质增生严重,见图6D1○;内部松质骨结构严重破坏变形,骨小梁大面积破损缺失,见图6D2→,且炎症已蔓延至整根胫骨,骨内膜有显著损伤,皮质骨增生且出现空隙,骨关节面破损,见图6D2○。"
骨体积是衡量骨骼形态的重要测量值,可以衡量骨骼的发育情况,与骨骼的力学性能也息息相关。随建模时间推移,胫骨整体骨体积均有所增加。对照组的整体骨体积为(1 548.1±251.0) mm3,建模2周的胫骨整体骨体积与对照组相比没有明显差异;建模4周胫骨有明显肿胀,整体骨体积相比对照组明显增大至(2 330.3±270.6) mm3(P < 0.01);建模6周,整体骨体积明显高于建模2周组和对照组(P < 0.001),见图7A。 皮质骨体积对照组和建模2周组相比无明显变化;而4周和6周皮质骨肿胀都较为明显,与2周建模和对照组相比差异均有显著性意义(P < 0.05),见图7B。 但由此次实验中的CT断层图像可见,骨骺线上下方松质骨均受炎症影响产生了较大变化,因此将其上下方的松质骨均纳入计算范围。在选取感兴趣区时,骨骺线下方仅选择骨小梁部分,而上方的松质骨与其表面较薄的皮质骨难以区分,因此也纳入了体积的统计。此次计算的松质骨体积是所有松质骨以及骨骺线上方骨表面直至髁间隆起处表层皮质骨的总体积。对照组松质骨骨体积为(172.7±44.6) mm3,2周松质骨体积略有增长至(228.6±120.4) mm3但与对照组相比差异无显著性意义。4周和6周松质骨体积与2周建模、对照组相比差异均有显著性意义(P < 0.05),见图7C。"
整体骨骨密度随建模时间变化不明显,约为930 mg/cm3。通过对皮质骨骨密度分析可见,建模组的皮质骨骨密度与对照组(1 000.4±26.4) mg/cm3相比,差异均有显著性意义(P < 0.05);但随着建模时间的延长,皮质骨骨密度无明显变化,分别为(928.3±19.5) mg/cm3(2周)、(941.8±70.5) mg/cm3(4周)以及(900.1±66.6) mg/cm3(6周)。松质骨在建模后的骨密度均明显高于对照组(691.2±55.2) mg/cm3(P < 0.05),但随建模时间的延长各组之间无明显变化,分别为(770.5±14.9) mg/cm3(2周)、(774.0±18.7) mg/cm3 (4周),直至6周时的(799.0±17.8) mg/cm3,见图8。"
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