Establishment and validation of C0-T1 finite element model of cervical vertebrae in adult rhesus monkeys

doi:10.12307/2021.292

Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (35): 5632-5637.doi: 10.12307/2021.292

Establishment and validation of C0-T1 finite element model of cervical vertebrae in adult rhesus monkeys

Wang Xing1, 2, Xu Xuebin2, Zhang Shaojie1, 2, Zheng Bingwu3, Yang Xi2, Wang Chaoqun4, Li Ping5, Ma Yuan6, Li Kun2, Chen Jie2, Li Xiaohe2, Shi Jun7, Li Zhijun1, 2

1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 101000, China; 2Department of Anatomy, 6Center for Digital Medicine, 7Department of Physiology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China

Received:2020-07-24 Revised:2020-08-18 Accepted:2020-10-16 Online:2021-12-18 Published:2021-08-03
Contact: Li Zhijun, Professor, Doctoral supervisor, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 101000, China; Department of Anatomy, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China
About author:Wang Xing, MD candidate, Lecturer, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 101000, China; Department of Anatomy, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China
Supported by:
the National Natural Science Foundation of China, No. 81860382 (to WX), 81860383, 81560348 (both to LZJ), and 81660358 (to ZSJ); Inner Mongolia Natural Science Foundation, No. 2020MS03061 (to WX), 2019MS08017 (to ZSJ), and 2019MS08139 (to CJ); Science and Technology Development Projects of Inner Mongolia Autonomous Region, No. 2019GG158 (to WX) and 2019GG111 (to LP); Science and Technology Million Project of Inner Mongolia Medical University, No. YKD2017KJBW009 (to WX) and 2015YKDKJBW03 (to ZSJ); Youth Innovation Fund of Inner Mongolia Medical University, No. TKD2018QNCX071 (to WCQ)

Abstract

Abstract: BACKGROUND: Both rhesus monkeys and humans belong to primates, and their cervical vertebra structure, physiological function and living conditions are very similar to those of human beings. However, there are few studies on the finite element analysis of the whole cervical vertebra of rhesus monkeys.
OBJECTIVE: To establish the C0-T1 finite element model of cervical vertebrae of adult rhesus monkeys and to make a comparative study between the finite element models of cervical vertebrae of adult rhesus monkeys and human cervical vertebrae in order to find out the difference.
METHODS: A 7-year-old adult male rhesus monkey was selected and scanned by multi-slice spiral CT in the Imaging Department of the affiliated Hospital of Inner Mongolia Medical University in December 2019. The original CT data of cervical vertebrae were introduced into Mimics21.0 to establish a preliminary three-dimensional model, and the geometric structure of the model was optimized by Cero to get the simulation three-dimensional model of cervical vertebrae. The assembly model was imported into Hypermesh for tetrahedral gridding to divide the tissue grids of various segments, intervertebral discs and ligaments of cervical vertebrae. Then the finite element model of cervical vertebra was constructed by Abaqus&ANSYS, and the validity was verified by referring to the relevant finite element model literature.
RESULTS AND CONCLUSION: The three-dimensional finite element model of cervical vertebra of rhesus monkey (including C0-T1 and six intervertebral discs and related ligaments) was successfully established, with a total of 536 215 elements and 461 915 nodes. The overall simulation of the model was high, and the fine structure was restored with high precision, resulting in a high biological simulation performance. Comparing the range of motion of this model with that of human cervical vertebra model, we found that the flexion and extension of this model were smaller than those of normal adults (P < 0.05), and left and right lateral bending of C2-3, C3-4 and C6-7 segments was smaller than the range of standard deviation as reported (P < 0.05). However, axial rotation showed no significant difference as compared with the value reported previously (P > 0.05). To conclude, there is a great difference in the exercise efficiency between the finite element models of rhesus monkey cervical vertebra and human cervical vertebra, which can provide a theoretical basis for the study of rhesus monkey cervical vertebra modeling.

Key words: adult rhesus monkey, whole cervical vertebra, finite element analysis, digital model, human

CLC Number:

Wang Xing, Xu Xuebin, Zhang Shaojie, Zheng Bingwu, Yang Xi, Wang Chaoqun, Li Ping, Ma Yuan, Li Kun, Chen Jie, Li Xiaohe, Shi Jun, Li Zhijun. Establishment and validation of C0-T1 finite element model of cervical vertebrae in adult rhesus monkeys[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(35): 5632-5637.

Figures/Tables 6

References

[1] 钟品仁,主编.哺乳类实验动物[M].北京:人民卫生出版社,1987: 317.
[2] ARORA T, ZHANG L, PRASAD P. Development of a Subhuman Primate Brain Finite Element Model to Investigate Brain Injury Thresholds Induced by Head Rotation. Stapp Car Crash J. 2019;63:65-82.
[3] GOEL A, KASWA A, SHAH A. Role of atlantoaxial and subaxial spinal instability in pathogenesis of spinal ‘degeneration’ related cervical kyphosis. World Neurosurg. 2017;101:702-709.
[4] WANG XD, FENG MS, HU YC. Establishment and Finite Element Analysis of a Three-dimensional Dynamic Model of Upper Cervical Spine Instability. Orthop Surg. 2019;11(3):500-509.
[5] 邵荣学,全仁夫,王拓,等.新型梯度复合HA/ZrO2组织工程骨支架在猕猴颈椎融合中的应用[J].中华解剖与临床杂志,2017,22(6): 499-509.
[6] BONO CM, MIN W. Avoiding complications in patients with ankylosing spondylitis undergoing spine surgery. Curr Opin Orthop. 2005;16(3): 178-183.
[7] SHIRAZI-ADL A, AHMED AM, SHRIVASTAVA SC, et al. Mechanical response of a lumbar motion segment in axial torque alone and combined with compression. Spine. 1986;11(9):914-927.
[8] SCHMIDT H, HEUER F, DRUMM J, et al. Application of a calibration method provides more realistic results for a finite element model of a lumbar spinal segment. Clin Biomech. 2007;22(4):377-384.
[9] PANJABI MM, CRISCO JJ, VASAVADA A, et al. Mechanical properties of the human cervical spine as shown by three-dimensional load-displacement curves. Spine. 2001;26(24):2692-2700.
[10] ITO S, IVANCIC PC, PANJABI MM, et al. Soft tissue injury threshold during simulated whiplash: a biomechanical investigation. Spine. 2004; 29(9):979-987.
[11] 陈强.挥鞭样损伤的生物力学和临床研究[J].第二军医大学学报, 2005,12(3):631-635.
[12] 郭群峰,陈方经,倪斌,等.带有颅底的全颈椎三维有限元模型的建立及分析[J].中国脊柱脊髓杂志,2014,24(6):550-554.
[13] 刘伟聪,陈雄生,周盛源,等.正常人体C0-T1全颈椎有限元模型的构建及意义[J].中国组织工程研究,2018,22(11):1707-1712.
[14] OLSZKO AV, BELTRAN CM, VASQUEZ KB, et al. Initial analysis of archived non-human primate frontal and rear impact data from the biodynamics data resource. Traffic Injury Prev. 2018;15(7):1165-1169.
[15] 凌泽莎.猕猴椎动脉及横突孔大水的CTA观察及其CSA模型的可行性研究[D].重庆:重庆医科大学,2014.
[16] LEE JH , PARK WM , KIM YH , et al. A Biomechanical Analysis of an Artificial Disc With a Shock-absorbing Core Property by Using Whole-cervical Spine Finite Element Analysis. Spine. 2016; 41(15):893-897.
[17] KIM YH, KHUYAGBAATAR B, KIM K. Recent advances in finite element modeling of the human cervical spine. J Mech Sci Technol. 2018;32(8): 1-10.
[18] BARKER JB, CRONIN DS, NIGHTINGALE RW. Lower Cervical Spine Motion Segment Computational Model Validation: Kinematic and Kinetic Response for Quasi-Static and Dynamic Loading. J Biomech Eng. 2017;139(6):610-613.
[19] LASSWELL TL, CRONIN DS, MEDLEY JB, et al. Incorporating ligament laxity in a finite element model for the upper cervical spine. Spine J. 2017;17(4):1755-1764.
[20] 凌泽莎,贾功伟,谭波涛,等.注射复合rhBMP-2骨水泥制作猕猴椎动脉型颈椎病模型[J].中山大学学报(医学科学版),2016,37(5): 775-780.
[21] CUNNINGHAM AS. Growth and Sexual Dimorphism of the Hyoid Body in Macaca mulatta. Springer US. 2020;28:178-181.
[22] ARORA T, ZHANG L, PRASAD P. Development of a Subhuman Primate Brain Finite Element Model to Investigate Brain Injury Thresholds Induced by Head Rotation. Stapp Car Crash J. 2019;63(3):65-82.
[23] 陈江波,潘希敏,陈应明,等. 磁共振T2 mapping和T1ρ成像研究群养成年恒河猴腰椎间盘的退变过程[J].中国组织工程研究, 2017,21(3):418-422.
[24] 汪韬,党耕町,郭昭庆,等. 自体骨髓基质干细胞与钙磷陶瓷复合体在恒河猴腰椎前路融合中的实验研究[J]. 中华外科杂志,2006, 44(12):843-847.
[25] 宋伟,赵文,魏瑞晗,等.脊髓损伤恒河猴后肢步态数据处理方法的设计[J]. 中国康复理论与实践,2013,19(8):734-738.
[26] 闵少雄,李森,刘成龙,等.恒河猴骨髓间充质干细胞体外培养及诱导分化成骨细胞的实验研究[J]. 中国矫形外科杂志,2011,19(3): 228-232.
[27] 孔杰,王子轩,季爱玉,等.应用微创技术建立恒河猴腰椎间盘早期退变模型[J].中华外科杂志,2008,46(11):835-838.
[28] 黄帆,邓忠良.部分可吸收椎间融合器应用于恒河猴腰椎融合的研究[D].重庆:重庆医科大学,2013.
[29] 凌泽莎,周志明,郑晓,等.实验用猕猴颈部骨骼和血管的影像学及血流动力学分析[J].中国实验动物学报,2015,23(5):500-505.
[30] WATSON DV , GANDHI AA , FREDERICKS DC , et al. Sheep cervical spine biomechanics: a finite element study. Iowa Orthop J. 2014;34:137-143.
[31] MENGONI M, VASILJEVA K, JONES AC, et al. Subject-specific multi-validation of a finite element model of ovine cervical functional spinal units. Biomech. 2016;49:259-266.
[32] 盛孙仁,徐华梓,王向阳,等.猪、小牛与人颈椎的生物力学比较[J].医用生物力学,2010,25(5):380-384.
[33] 薛德明.太行山猕猴寰椎和枢椎的初步研究[J].动物学杂志,2003, 9(2):74-75.
[34] 范春梅,李志雄,周建华.实验猕猴头颈部CT影像学观察[J].畜禽业,2012,10(4):30-32.
[35] 肖莉,杨红,石清明,等.藏酋猴脊柱的解剖学研究[J].西南国防医药,2017,5(10):1037-1040.
[36] SPARREY CJ, SALEGIO EA, CAMISA W, et al. Mechanical Design and Analysis of a Unilateral Cervical Spinal Cord Contusion Injury Model in Non-Human Primates. J Neurotrauma. 2016;33(12):1136-1149.
[37] LING Z, LI L, CHEN Y, et al. Changes of the end plate cartilage are associated with intervertebral disc degeneration:A quantitative magnetic resonance imaging study in rhesus monkeys and humans. J Orthop Translat. 2020;24:23-31.

Establishment and validation of C0-T1 finite element model of cervical vertebrae in adult rhesus monkeys

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Lin Qingfan, Xie Yixin, Chen Wanqing, Ye Zhenzhong, Chen Youfang. Human placenta-derived mesenchymal stem cell conditioned medium can upregulate BeWo cell viability and zonula occludens expression under hypoxia [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(在线): 4970-4975.
[2]	Chen Xinmin, Li Wenbiao, Xiong Kaikai, Xiong Xiaoyan, Zheng Liqin, Li Musheng, Zheng Yongze, Lin Ziling. Type A3.3 femoral intertrochanteric fracture with augmented proximal femoral nail anti-rotation in the elderly: finite element analysis of the optimal amount of bone cement [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(9): 1404-1409.
[3]	Duan Liyun, Cao Xiaocang. Human placenta mesenchymal stem cells-derived extracellular vesicles regulate collagen deposition in intestinal mucosa of mice with colitis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1026-1031.
[4]	Cai Qunbin, Zou Xia, Hu Jiantao, Chen Xinmin, Zheng Liqin, Huang Peizhen, Lin Ziling, Jiang Ziwei. Relationship between tip-apex distance and stability of intertrochanteric femoral fractures with proximal femoral anti-rotation nail: a finite element analysis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(6): 831-836.
[5]	Song Chengjie, Chang Hengrui, Shi Mingxin, Meng Xianzhong. Research progress in biomechanical stability of lateral lumbar interbody fusion [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(6): 923-928.
[6]	Liu Zhao, Xu Xilin, Shen Yiwei, Zhang Xiaofeng, Lü Hang, Zhao Jun, Wang Zhengchun, Liu Xuzhuo, Wang Haitao. Guiding role and prospect of staging and classification combined collapse prediction method for osteonecrosis of femoral head [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(6): 929-934.
[7]	Nie Huijuan, Huang Zhichun. The role of Hedgehog signaling pathway in transforming growth factor beta1-induced myofibroblast transdifferentiation [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 754-760.
[8]	Yang Jiujie, Wang Tao, Li Zhi, Yang Lifeng, Tian Ye, Bi Zheng, Zeng Yaling. Cervical spondylosis with osteoporosis treated by the new pedicle fixation system through the anterior cervical approach with three-dimensional printing technology: three-dimensional finite element analysis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5249-5253.
[9]	Gu Jinshan, Yang Chaohui, Li Shuwei. Mechanical difference between anterior approach and anterior combined with posterior approach in the treatment of acetabular both-column fractures [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5254-5258.
[10]	Xie Jiang, Guo Huili, Li Hui, Dai Jie, Zhu Xu. Finite element analysis of reconstruction of sagittal balance in ankylosing kyphosis with vertebral column resection [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5259-5264.
[11]	Wu Chao, Gao Mingjie, Wang Jianzhong, Zhang Yunfeng, Yu Jinghong, Cai Yongqiang, Wang Haiyan, He Yujie, Tong Ling, Li Jiawei, Gao Shang, Wang Xing, Wu Min, Li Zhijun, Li Xiaohe. Finite element analysis of stress and displacement of Lenke 3 adolescent idiopathic scoliosis thoracolumbar spine [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5273-5280.
[12]	Lü Jie, Wang Yongfeng, Yuan Jie, Xu Zhaojian, Qin Yichuan, Hao Jiaqi. Biomechanical finite element analysis of lateral displacement of the cage after oblique lumbar interbody fusion [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5301-5306.
[13]	Zhang Wen, Chen Liang, Jiang Zhenhuan. Biomechanical characteristics of bone cement reduction for V-type calcaneal fractures [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5307-5311.
[14]	Hou Kunpeng, Zhao Quanlai, Wu Tianliang, Xiao Liang, Liu Chen, Xu Hongguang. Biomechanical stability of oblique lateral lumbar fusion and internal fixation [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(33): 5362-5368.
[15]	Yi Zhishuang, Wang Zhihai, Li Ming, Zhou Qingan, Yang Baosheng. Exosomes from human umbilical cord-derived mesenchymal stem cells suppress the proliferation and promote the apoptosis of medulloblastoma Daoy cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 4945-4949.