Poly(lactide-co-glycolide) scaffold combined with adipose derived stem cells in tissue-engineered urethral reconstruction

doi:10.12307/2021.243

Abstract

Abstract: BACKGROUND: The combination of bioelectrospray and electrospinning can promote the direct integration of living cells and scaffold materials, and can distribute the cells evenly among scaffold fibers, which is a promising alternative to the preparation of scaffold containing cells.
OBJECTIVE: To investigate the feasibility using multiple methods to prepare poly(lactide-co-glycolide) (PLGA) three-dimensional scaffolds rich in adipose derived stem cells (ASCs) as urethral tissue reconstruction materials.
METHODS: The ASCs were integrated into PLGA by electrospinning and cell bio-electrospray method. The cell scaffold PLGA-ASCs was obtained. The pure PLGA scaffold was prepared by electrospinning. The microstructure, in vitro degradation, mechanical properties and residual solvent content of the two scaffolds were detected. The cell viability of PLGA-ASCs was detected by MTT assay. PLGA-ASCs were cultured in 37°C cell incubator for 1, 7 and 15 days. MTT assay was used to detect cell viability. Scanning electron microscopy and confocal microscopy were used to observe the growth and diffusion of ASCs on the scaffolds.
RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that the fiber surface of PLGA-ASCs and PLGA scaffolds was smooth, and ASCs were randomly distributed on PLGA-ASCs scaffolds. The average fiber diameter and thickness of PLGA-ASCs scaffolds were larger than those of PLGA scaffolds. The cell survival rate in PLGA-ASCs scaffolds was (87.0±4.4)%, and the cell integration efficiency in PLGA-ASCs scaffolds was 28%. (2) In vitro degradation experiment showed that weight average molecular weight of PLGA scaffolds decreased rapidly in the first 15 days. The weight average molecular weight of PLGA-ASCs scaffolds decreased rapidly on 15-45 days. There was no difference in weight average molecular weight between the two groups at 45 days. (3) Young’s modulus, maximum load, and maximum elongation of PLGA-ASCs scaffolds were lower than those of PLGA scaffolds. (4) During 1-7 days of culture, the number of cells in PLGA-ASCs scaffolds gradually increased, and the number of cells in PLGA-ASCs scaffolds increased gradually from 7 to 15 days. (5) The results of scanning electron microscope observation and confocal microscope showed that the number of cells in PLGA-ASCs scaffolds increased gradually and integrated with the scaffolds with the extension of culture time. (6) The results showed that PLGA-ASCs had good physical and chemical properties and biological activity, and could be used as urethral tissue reconstruction materials.

Key words: material, biological scaffold, poly(lactide-co-glycolide), electrospinning, bioelectrospray, adipose, stem cells, integration

CLC Number:

Li Zuowei, Fu Qiang, Song Lujie, Li Yonghui, Tian Binqiang. Poly(lactide-co-glycolide) scaffold combined with adipose derived stem cells in tissue-engineered urethral reconstruction[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(34): 5484-5489.

Figures/Tables 8

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