中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (8): 2001-2013.doi: 10.12307/2026.071

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

二甲双胍联合血水草敷料治疗深Ⅱ度烧伤创面的作用机制

董春阳1，周天恩1，莫孟学2，吕文权3，高  明2，朱瑞凯3，高志伟2   

  1. 1广东省佛山市第一人民医院，广东省佛山市   528000；2广西医科大学生命科学研究院，广西壮族自治区南宁市   530000；3中山大学附属第一医院广西医院，广西壮族自治区南宁市   530000
  • 收稿日期:2024-11-15 接受日期:2025-02-06 出版日期:2026-03-18 发布日期:2025-07-17
  • 通讯作者: 高志伟，医师，广西医科大学生命科学研究院，广西壮族自治区南宁市 530000 朱瑞凯，医师，中山大学附属第一医院广西医院，广西壮族自治区南宁市 530000
  • 作者简介:董春阳，女，1985年生，广东省佛山市人，汉族，硕士，主治医师，主要从事急危重症的临床研究分析。 周天恩，男，1979年生，福建省晋江市人，汉族，博士，主任医师，主要从事急诊医学研究。
  • 基金资助:
    中山大学第一附属医院广西医院青年科学基金项目(YQ2024026)，项目负责人：吕文权

Action mechanism of metformin combined with Eomecon chionantha Hance dressing in treatment of deep second-degree burn wounds#br#
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Dong Chunyang1, Zhou Tianen1, Mo Mengxue2, Lyu Wenquan3, Gao Ming2, Zhu Ruikai3, Gao Zhiwei2   

  1. 1First People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China; 2Life Sciences Research Institute, Guangxi Medical University, Nanning 530000, Guangxi Zhuang Autonomous Region, China; 3Guangxi Hospital Division of The First Affiliated Hospital of Sun Yat-sen University, Nanning 530000, Guangxi Zhuang Autonomous Region, China
  • Received:2024-11-15 Accepted:2025-02-06 Online:2026-03-18 Published:2025-07-17
  • Contact: Gao Zhiwei, Physician, Life Sciences Research Institute, Guangxi Medical University, Nanning 530000, Guangxi Zhuang Autonomous Region, China Zhu Ruikai, Physician, Guangxi Hospital Division of The First Affiliated Hospital of Sun Yat-sen University, Nanning 530000, Guangxi Zhuang Autonomous Region, China
  • About author:Dong Chunyang, MS, Attending physician, First People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China Zhou Tianen, MD, Chief physician, First People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China
  • Supported by:
    Youth Science Fund of Guangxi Hospital Division of The First Affiliated Hospital of Sun Yat-sen University, No. YQ2024026 (to LWQ)

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摘要：

文题释义：
血水草：主要活性成分包括生物碱、黄酮类和酚酸类化合物，具有清热解毒、活血化瘀等功效，主治跌打损伤和急慢性疮疡。
二甲双胍：是治疗Ⅱ型糖尿病的首选和全程药物，但不仅限于治疗糖尿病，在肿瘤、炎症和多种自身免疫性疾病中也发挥重要作用。

背景：二甲双胍和血水草均具有抗炎和血管保护作用，其中二甲双胍可通过改善氧化应激等多种方式促进组织修复。
目的：探讨二甲双胍联合血水草对深Ⅱ度烧伤创面的抑菌抗炎促修复作用。
方法：①体外实验：检测血水草、血水草+低剂量二甲双胍(10 mmol/L)、血水草+高剂量二甲双胍(100 mmol/L)对过氧化氢诱导RAW264.7细胞(或L929细胞)内活性氧水平的影响。检测血水草、血水草+低剂量二甲双胍(10 mmol/L)、血水草+高剂量二甲双胍(100 mmol/L)对L929细胞划痕愈合能力的影响。检测血水草、血水草+低剂量二甲双胍(10 mmol/L)、血水草+高剂量二甲双胍(100 mmol/L)对脂多糖诱导RAW264.7细胞内磷酸化核因子κB p65、核因子κB p65蛋白表达的影响。通过试管二倍稀释法联合琼脂稀释涂布平板法与细菌活死染色检测血水草、血水草+低剂量二甲双胍(10 mmol/L)、血水草+高剂量二甲双胍(100 mmol/L)对金黄色葡萄球菌的抑制作用。②动物体内实验：通过SD大鼠肝脏裂口模型、断尾模型观察血水草的止血性能。取16只SD大鼠，每只背部建立4个直径为2 cm的深Ⅱ度烧伤创面，造模4 h后将金黄色葡萄球菌悬液涂抹于创面。菌液涂抹24 h后分4组干预，每组4只：造模组皮下注射生理盐水，创面用浸润生理盐水的纱布湿敷；血水草组创面用浸润血水草的纱布湿敷；低剂量二甲双胍血水草组皮下注射10 mmol/L二甲双胍1 mL，创面用浸润血水草的纱布湿敷；高剂量二甲双胍血水草组皮下注射100 mmol/L二甲双胍1 mL，创面用浸润血水草的纱布湿敷，皮下给药时间为第1，2，4，6天，每天更换纱布。给药后7 d内，观察创面愈合、创面组织形态、创面氧化应激指标、炎症因子与血管新生相关蛋白表达。
结果与结论：①相较于单独的血水草，二甲双胍联合血水草具有更强的活性氧清除能力、抗菌能力、促细胞迁移能力以及抑制核因子κB炎症信号通路的作用，并且以血水草联合高剂量二甲双胍的作用更明显。②血水草具有良好的止血效果，可缩短止血时间、减少出血量。相较于单独的血水草，二甲双胍联合血水草可更好地促创面愈合、改善创面组织形态、抑制炎症反应与氧化应激反应，提高血管内皮生长因子蛋白表达，并且以血水草联合高剂量二甲双胍的作用更明显。③结果表明，二甲双胍联合血水草可通过抗菌、抗炎、抗氧化及促细胞迁移促进深Ⅱ度烧伤创面的修复。
https://orcid.org/0009-0004-9609-7229 (董春阳) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 二甲双胍, 血水草, 烧伤, 创面愈合, 氧化应激, 深Ⅱ度烧伤, 工程化皮肤材料

Abstract: BACKGROUND: Metformin and Eomecon chionantha Hance both exhibit anti-inflammatory and vascular protective properties. Metformin has been proven to promote tissue repair through various mechanisms, such as reducing oxidative stress. 
OBJECTIVE: To investigate the antibacterial, anti-inflammatory, and wound-healing effects of metformin combined with Eomecon chionantha Hance on deep second-degree burn wounds.
METHODS: (1) In vitro experiments: The effects of Eomecon chionantha Hance, Eomecon chionantha Hance + low-dose metformin (10 mmol/L), and Eomecon chionantha Hance + high-dose metformin (100 mmol/L) on the level of reactive oxygen species in RAW264.7 cells (or L929 cells) induced by hydrogen peroxide were detected. The effects of Eomecon chionantha Hance, Eomecon chionantha Hance + low-dose metformin (10 mmol/L), and Eomecon chionantha Hance + high-dose metformin (100 mmol/L) on the scratch healing ability of L929 cells were detected. The effects of Eomecon chionantha Hance, Eomecon chionantha Hance + low-dose metformin (10 mmol/L), and Eomecon chionantha Hance + high-dose metformin (100 mmol/L) on the expression of phosphorylated nuclear factor κB p65 and nuclear factor κB p65 protein in RAW264.7 cells induced by lipopolysaccharide were detected. The inhibitory effects of Eomecon chionantha Hance, Eomecon chionantha Hance + low dose metformin (10 mmol/L), and Eomecon chionantha Hance + high dose metformin (100 mmol/L) on Staphylococcus aureus were detected by test tube double dilution method combined with agar dilution plate method and bacterial live and dead staining. (2) In vivo animal experiment: The hemostatic properties of Eomecon chionantha Hance were observed by liver laceration model and tail amputation model of SD rats. 16 SD rats were selected, and 4 deep second-degree burn wounds with a diameter of 2 cm were established on the back of each rat. Staphylococcus aureus suspension was applied to the wound 4 hours after modeling. 24 hours after the bacterial liquid was applied, the rats were divided into four groups for intervention, with four rats in each group: the modeling group was injected with normal saline subcutaneously, and the wound was wetted with gauze soaked in normal saline. The Eomecon chionantha Hance group was wetted with gauze soaked in Eomecon chionantha Hance. The low-dose metformin Eomecon chionantha Hance group was injected with 10 mmol/L metformin 1 mL subcutaneously, and the wound was wetted with gauze soaked in Eomecon chionantha Hance. The high-dose metformin Eomecon chionantha Hance group was injected with 100 mmol/L metformin 1 mL subcutaneously, and the wound was wetted with gauze soaked in Eomecon chionantha Hance. The subcutaneous administration time was 1, 2, 4, and 6 days, and the gauze was changed every day. Within 7 days after administration, the wound healing, wound tissue morphology, wound oxidative stress indicators, inflammatory factors, and angiogenesis-related protein expressions were observed. 
RESULTS AND CONCLUSION: (1) Compared with the Eomecon chionantha Hance alone, metformin combined with Eomecon chionantha Hance had stronger reactive oxygen scavenging ability, antibacterial ability, cell migration promotion ability and inhibition of nuclear factor κB inflammatory signaling pathway, and the effect of Eomecon chionantha Hance combined with high-dose metformin was more obvious. (2) Eomecon chionantha Hance had a good hemostatic effect, which could shorten the hemostatic time and reduce the amount of bleeding. Compared with Eomecon chionantha Hance alone, metformin combined with Eomecon chionantha Hance could better promote wound healing, improve wound tissue morphology, inhibit inflammatory response and oxidative stress response, and increase vascular endothelial growth factor protein expression, and the effect of Eomecon chionantha Hance combined with high-dose metformin was more obvious. (3) The results show that metformin combined with Eomecon chionantha Hance can promote the repair of deep second-degree burn wounds through antibacterial, anti-inflammatory, antioxidant, and cell migration promotion. 


Key words: metformin, Eomecon chionantha Hance, burn, wound healing, oxidative stress, deep second-degree burn, engineered skin material

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