关键词: 高压氧, 高强度间歇冲击微周期, 运动性疲劳, 氧化应激, 代谢组学

Abstract: BACKGROUND: Hyperbaric oxygen, as one of the emerging means of fatigue elimination, has been increasingly valued and applied in the field of sports. However, there are fewer studies on the effect of hyperbaric oxygen intervention on fatigue elimination after high-intensity interval training shock microcycle.
OBJECTIVE: To investigate the effect of hyperbaric oxygen intervention on the elimination of exercise-induced fatigue in the high-intensity interval training shock microcycle, and to study the corresponding mechanisms in terms of blood biochemical markers and metabolomics.
METHODS: Twenty male college students were recruited from the Capital University of Physical Education and Sports, and randomly divided into a control group (n=10) and a hyperbaric oxygen group (n=10). Both groups underwent high-intensity interval training shock microcycle training for 2 weeks, a total of 12 sessions, with the following specific training program: warming up at 50% of the maximum heart rate for 10 minutes, and then pedaling at 90%-95% of the maximum heart rate for 4 minutes, repeating the program for 5 sessions, with a rest period of 2.5 minutes in between sessions, and finally pedaling at 50% of the maximum heart rate for 30 minutes. Subjects in the control groups recovered naturally after training, and those in the hyperbaric group recovered from training with hyperbaric oxygen, 60 minutes each, at a pressure of 131.722 kPa. Blood biochemical markers and metabolomics data were analyzed and rating of perceived exertion was performed before, during and at 1 and 3 days after the experiment. Oxidative stress indicators and fatigue monitoring indicators were analyzed by Pearson correlation analysis. 
RESULTS AND CONCLUSION: (1) Regarding exercise-induced fatigue indicators, the control group showed varying degrees of increase in blood uric acid, creatine kinase, interleukin 6 and the rating of perceived exertion after training, while the hyperbaric oxygen group exhibited minimal changes in blood uric acid, creatine kinase, interleukin 6 and the rating of perceived exertion after training. Additionally, blood uric acid, creatine kinase, and interleukin 6 levels in the control group were significantly higher than those in the hyperbaric oxygen group at 1 day after training. (2) In the control group, superoxide dismutase levels decreased, while malondialdehyde levels increased after training. Conversely, in the hyperbaric oxygen group, superoxide dismutase levels increased, while malondialdehyde levels decreased after training. (3) Superoxide dismutase levels showed a negative correlation with blood uric acid, interleukin 6 and the rating of perceived exertion, while malondialdehyde levels exhibited a positive correlation with interleukin 6 and the rating of perceived exertion. (4) In the metabolomics analysis, significant changes were observed in the metabolic pathways of arachidonic acid metabolism and oxidative phosphorylation. Differential metabolites enriched in these pathways included arachidonic acid, prostaglandin D2, leukotriene D4, etc. To conclude, the high-intensity interval training shock microcycle induces oxidative stress, leading to exercise-induced fatigue in the body. Hyperbaric oxygen intervention can partially ameliorate oxidative stress levels and cause arachidonic acid metabolism and oxidative phosphorylation, thereby reducing oxidative damage, regulating inflammatory responses, promoting tissue repair, and alleviating exercise-induced fatigue.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: hyperbaric oxygen, high-intensity interval training shock microcycle, exercise-induced fatigue, oxidative stress, metabolomics