中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (5): 958-967.doi: 10.12307/2025.203

• 脊柱组织构建 spinal tissue construction • 上一篇    下一篇

创伤性脊髓损伤急性期前列腺素E1对血管相关因子的调节和微循环功能的保护

王荣荣1，2，黄玉珊1，2，李湘淼1，2，白金柱1，2，3   

  1. 1首都医科大学康复医学院，北京市  100068；2中国康复研究中心北京博爱医院脊柱脊髓外科，北京市  100068；3首都医科大学骨外科学系，北京市  100069
  • 收稿日期:2023-11-29 接受日期:2024-01-20 出版日期:2025-02-18 发布日期:2024-06-03
  • 通讯作者: 白金柱，博士，主任医师，首都医科大学康复医学院，北京市 100068；中国康复研究中心北京博爱医院脊柱脊髓外科，北京市 100068；首都医科大学骨外科学系，北京市 100069
  • 作者简介:王荣荣，女，1998年生，河南省永城市人，汉族，2024年首都医科大学毕业，硕士，主要从事脊柱脊髓损伤与康复方面的研究。
  • 基金资助:
    中国康复研究中心重点项目(2022ZX-05，2018ZX-08)，项目负责人：白金柱

Prostaglandin E1 regulates vascular-related factors and protects microcirculatory function during the acute phase of traumatic spinal cord injury 

Wang Rongrong1, 2, Huang Yushan1, 2, Li Xiangmiao1, 2, Bai Jinzhu1, 2, 3   

  1. 1School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China; 2Department of Spine and Spinal Cord Surgery, Beijing Bo’ai Hospital, China Rehabilitation Research Center, Beijing 100068, China; 3Department of Orthopedics, Capital Medical University, Beijing 100069, China
  • Received:2023-11-29 Accepted:2024-01-20 Online:2025-02-18 Published:2024-06-03
  • Contact: Bai Jinzhu, MD, Chief physician, School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China; Department of Spine and Spinal Cord Surgery, Beijing Bo’ai Hospital, China Rehabilitation Research Center, Beijing 100068, China; Department of Orthopedics, Capital Medical University, Beijing 100069, China
  • About author:Wang Rongrong, Master, School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China; Department of Spine and Spinal Cord Surgery, Beijing Bo’ai Hospital, China Rehabilitation Research Center, Beijing 100068, China

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摘要：




文题释义：
脂质前列腺素E1：以脂微球为药物载体的静脉注射用前列地尔制剂，由于脂微球的包裹，前列地尔不易失活，且具有易于分布到受损血管部位的靶向特性，发挥药物扩张血管、抑制血小板聚集的作用。
微循环障碍：指微循环水平的形态和功能紊乱，在致病因素作用下，出现全身或局部性的微循环灌流与组织需要不相适应，灌流量明显降低，组织缺血、缺氧，组织代谢障碍，进一步则出现组织变性坏死、 功能衰竭等一系列变化。
血管失衡：指的是血管系统中的一种紊乱状态，其中血管的正常功能受到影响，导致血液流动、血管张力、血管壁通透性等方面的异常变化。血管失衡主要表现为血流调节异常、血管通透性改变、炎症反应、血栓形成、血压调节问题。

背景：前列腺素E1被证明在血管扩张、炎症、白细胞迁移和黏附中发挥调节作用，但其对创伤性脊髓损伤后脊髓微循环的作用尚缺乏深入的研究。
目的：探讨在大鼠创伤性脊髓损伤急性期给予前列腺素E1对血管相关因子的调节和微循环功能的保护作用机制。
方法：将72只雌性SD大鼠随机分为3组(n=24)，即假手术组、脊髓损伤组、前列腺素E1组。后两组用Allen’s 打击法建立脊髓损伤的体内模型，前列腺素E1组大鼠在脊髓损伤后15 min内立即尾静脉注射脂质前列腺素E110 µg/kg。分别在损伤后2，24 h测定脊髓微循环血流量和血氧饱和度、脊髓微血管直径和面积、脊髓含水量、血管功能调节因子(血浆血管性血友病因子、血栓素A2、前列环素、内皮素1)和炎症因子(肿瘤坏死因子α、白细胞介素1β)的表达。
结果与结论：①脊髓损伤后2 h，前列腺素E1组大鼠的脊髓微血管直径及面积、脊髓微循环血流量和血氧饱和度均高于脊髓损伤组(P < 0.05)，脊髓含水量低于脊髓损伤组(P < 0.05)，血浆血管性血友病因子、脊髓组织血栓素A2/前列环素及内皮素1质量浓度均低于脊髓损伤组(P < 0.05)；②脊髓损伤后24 h，前列腺素E1组大鼠的脊髓微血管面积、血流量和血氧饱和度均高于脊髓损伤组(P < 0.05)，脊髓含水量低于脊髓损伤组(P < 0.05)，血浆血管性血友病因子、脊髓组织血栓素A2/前列环素及内皮素1 、肿瘤坏死因子α、白细胞介素1β的质量浓度均低于脊髓损伤组(P < 0.05)；③脊髓损伤组大鼠损伤后24 h的脊髓微血管直径及面积、脊髓微循环血流量和血氧饱和度均高于损伤后2 h
(P < 0.05)，血浆血管性血友病因子、脊髓组织血栓素A2/前列环素、肿瘤坏死因子α、白细胞介素1β的质量浓度均高于损伤后2 h(P < 0.05)，但是脊髓组织内皮素1质量浓度低于损伤后2 h(P < 0.05)；④前列腺素E1组大鼠损伤后24 h的脊髓微循环血流量和血氧饱和度低于损伤后2 h(P < 0.05)，脊髓微血管直径及面积、脊髓含水量高于损伤后2 h(P < 0.05)；⑤以上结果表明，脊髓损伤大鼠伤后即刻静脉给予前列腺素E1，可调节血管功能调节因子、炎症因子并改善脊髓损伤后脊髓微循环，这为寻找治疗急性脊髓损伤的药物提供了潜在的基础。
https://orcid.org/0009-0004-3242-3961(王荣荣)


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 脊髓损伤, 前列腺素E1, 脊髓微循环, 微循环障碍, 炎症因子, 血管功能调节因子

Abstract: BACKGROUND: Prostaglandin E1 (PGE1) has been shown to play a regulatory role in vasodilatation, inflammation, and leukocyte migration and adhesion, but its effects on spinal cord microcirculation after traumatic spinal cord injury (SCI) remain poorly understood.
OBJECTIVE: To investigate the mechanism underlying the protective effects of PGE1 administered during the acute phase of traumatic SCI in rats on the regulation of vascular-related factors and microcirculatory function.
METHODS: Seventy-two female Sprague-Dawley rats were divided into three groups (n=24 per group): control group, SCI group, and PGE1 group. An in vivo SCI model was established using Allen’s blow method. Rats in the PGE1 group were injected with PGE1 (10 µg/kg) via the tail vein immediately after SCI. Spinal cord microcirculatory blood flow and oxygen saturation, spinal cord microvessel diameter and area, spinal cord water content, vascular function regulators (von Willebrand factor, thromboxane A2, prostacyclin, endothelin-1), and inflammatory factors (tumor necrosis factor-α, interleukin-1β) were measured at 2 and 24 hours after SCI.
RESULTS AND CONCLUSION: At 2 hours after SCI, the diameter and area of spinal cord microvessels, spinal cord microcirculatory blood flow, and oxygen saturation in the PGE1 group were higher than those in the SCI group (P < 0.05), the water content of the spinal cord was lower than that in the SCI group 
(P < 0.05), and the level of plasma von Willebrand Factor, the ratio of thromboxane A2/prostacyclin of the spinal cord and the level of endothelin-1 were lower than those in the SCI group (P < 0.05). At 24 hours after SCI, the spinal cord microvessel area, blood flow, and oxygen saturation of rats in the PGE1 group were higher than those in the SCI group (P < 0.05), the spinal cord water content was lower than that in the SCI group (P < 0.05), and the levels of plasma von Willebrand factor, spinal cord tissue thromboxane A2/prostacyclin ratio and the levels of endothelin-1, tumor necrosis factor-α and interleukin-1β were lower than those in the SCI group (P < 0.05). The diameter and area of spinal cord microvessels, spinal cord microcirculatory blood flow and blood oxygen saturation of rats in the SCI group were higher than those in the SCI group at 24 hours post-injury (P < 0.05), and the levels of plasma von Willebrand factor, spinal tissue thromboxane A2/prostacyclin ratio, tumor necrosis factor-α and interleukin-1β were higher than those at 2 hours post-injury (P < 0.05), but the level of endothelin-1 in spinal cord tissue was lower than that at 2 hours (P < 0.05). The blood flow and oxygen saturation of spinal cord microcirculation in the PGE1 group rats at 24 hours post-injury were lower than those at 2 hours post-injury (P < 0.05), and the diameter and area of spinal cord microvessels and water content of the spinal cord were higher than those at 2 hours post-injury (P < 0.05). The above results indicate that intravenous administration of PGE1 in SCI rats immediately after injury can regulate vascular function regulators, inflammatory factors and improve microcirculation of the spinal cord after SCI, which provides a potential basis for the search of drugs for the treatment of acute SCI.


Key words: spinal cord injury, prostaglandin E1, spinal cord microcirculation, microcirculatory disorder, inflammatory factor, vascular function regulator

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