中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (31): 6601-6608.doi: 10.12307/2025.673

• 骨髓干细胞 bone marrow stem cells •    下一篇

鹿角片煅制品促进骨髓间充质干细胞的增殖

邵学坤1，石典花2，3，丁志坪1，邱卓雅1，王  平2，王  仪2，王  成2，丁晓彦2，孙铁锋1，2   

  1. 1山东中医药大学，山东省济南市  250355；2山东省中医药研究院，山东省济南市  250014；3国家中医药管理局中药蜜制和制炭炮制技术与原理重点研究室，山东省济南市  250014
  • 收稿日期:2024-07-02 接受日期:2024-08-13 出版日期:2025-11-08 发布日期:2025-02-17
  • 通讯作者: 王平，博士，研究员，山东省中医药研究院，山东省济南市 250014； 共同通讯作者：石典花，博士，研究员，山东省中医药研究院，山东省济南市 250014；国家中医药管理局中药蜜制和制炭炮制技术与原理重点研究室，山东省济南市 250014
  • 作者简介:邵学坤，男，1997年生，山东省济宁市人，汉族，硕士，主要从事药理学研究。
  • 基金资助:
    山东省重点研发计划(科技示范工程)(2021SFGC1205)，项目负责人：王平；济南市“新高校20条”资助项目引进创新团队计划(202333006)，项目负责人：曹鹏；济南市“新高校20条”资助项目(202228121)，项目负责人：王平；泰山学者工程资助(tstp20231239)，项目负责人：曹鹏

Calcined deer antler slices promote proliferation of bone marrow mesenchymal stem cells

Shao Xuekun1, Shi Dianhua2, 3, Ding Zhiping1, Qiu Zhuoya1, Wang Ping2, Wang Yi2, Wang Cheng2, Ding Xiaoyan2, Sun Tiefeng1, 2   

  1. 1Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China; 2Shandong Institute of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; 3The key Unit for Research of Technique and Principle of Honey-processing and Carbonizing of SATCM, Jinan 250014, Shandong Province, China
  • Received:2024-07-02 Accepted:2024-08-13 Online:2025-11-08 Published:2025-02-17
  • Contact: Wang Ping, MD, Researcher, Shandong Institute of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; Co-corresponding author: Shi Dianhua, MD, Researcher, Shandong Institute of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; The key Unit for Research of Technique and Principle of Honey-processing and Carbonizing of SATCM, Jinan 250014, Shandong Province, China
  • About author:Shao Xuekun, MS, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China
  • Supported by:
    Shandong Provincial Key R&D Program (Science and Technology Demonstration Project), No. 2021SFGC1205 (to WP); Jinan City “New Colleges and Universities 20” Funding Project Introduction Innovation Team Plan, No. 202333006 (to CP); Jinan City “New Colleges and Universities 20” Funding Project, No. 202228121 (to WP); Taishan Scholars Project Funding, No. tstp20231239 (to CP) 

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摘要：

文题释义：

煅制：是将药物直接放于无烟炉火或适当的耐火容器中煅烧的一种炮制方法。按煅制方法的不同，可分为明煅、暗煅和煅淬3种。中药经煅制后，大部分会变酥脆，便于粉碎，利于有效成分煎出。煅制还可减轻某些药物的刺激性，降低毒性，改变或增强药效。
细胞增殖：是指细胞通过分裂过程产生新细胞的一种生物学现象，是生物体生长、修复和维持的重要机制。细胞增殖主要包括两个连续的过程：细胞生长和细胞分裂。在生长阶段，细胞积累必需的分子和能量，为分裂做准备。随后，细胞通过有丝分裂或无丝分裂的方式进行分裂。有丝分裂是最常见的形式，确保了遗传物质的精确复制和均等分配，从而使每个新生细胞都具有与母细胞相同的遗传信息。

摘要
背景：通过对鹿角片煅制品促进骨髓间充质干细胞增殖效果的科学探究，为传统中医药与现代再生医学的融合创新提供实证支持，推进传统中药在骨骼系统疾病治疗领域的广泛应用。
目的：探讨鹿角片煅制品对骨髓间充质干细胞增殖的影响。
方法：通过不同材料(如陶泥、黄泥、盐黄泥)的包裹，对鹿角片进行煅制处理，共设计7种不同的煅制样品(陶泥-棉布、黄泥-棉布、盐黄泥-棉布、黄泥-锡箔纸、盐黄泥-锡箔纸、黄泥-蜜炙、盐黄泥-蜜炙煅制的鹿角片)，测定煅制前后鹿角片水溶性浸出物含量。采用CCK-8实验评估不同鹿角片煅制品水提物对骨髓间充质干细胞增殖活性的影响。
结果与结论：①煅制方法显著提高了鹿角片的水溶性浸出物含量，其中黄泥-蜜炙煅制鹿角片的水溶性浸出物含量最高；②鹿角片煅制品能够显著促进骨髓间充质干细胞的增殖，其中黄泥-蜜炙煅制鹿角片对骨髓间充质干细胞增殖的促进效果最为显著。

关键词: 骨髓间充质干细胞, 细胞增殖, 鹿角片, 煅制, 黄泥, 蜜炙, 水提物, 工程化干细胞

Abstract: BACKGROUND: Through scientific research addressing the effect of calcined deer antler slices on promoting the proliferation of bone marrow mesenchymal stem cells, it aims to provide empirical support for the integration and innovation of traditional Chinese medicine and modern regenerative medicine, and promote the widespread application of traditional Chinese medicine in the treatment of skeletal system diseases.
OBJECTIVE: To investigate the effect of calcined deer antler slices on bone marrow mesenchymal stem cell proliferation. 
METHODS: Different calcination samples were prepared by wrapping deer antler slices with materials such as clay, yellow clay, and salted yellow clay, resulting in seven different samples (clay-cotton cloth, yellow clay-cotton cloth, salted yellow clay-cotton cloth, yellow clay-tin foil, salted yellow clay-tin foil, yellow clay-honey roasted, salted yellow clay-honey roasted antler slices). Water-soluble extract content in deer antler slices was determined before and after calcination. CCK-8 assay was used to evaluate the effects of different aqueous extracts of calcined antler slices on the proliferation activity of bone marrow mesenchymal stem cells.
RESULTS AND CONCLUSION: (1) Calcination significantly increased the water-soluble extract content of deer antler slices, with the highest content observed in samples treated with yellow clay and honey. (2) Calcined deer antler slices significantly promoted bone marrow mesenchymal stem cell proliferation, among which the yellow clay-honey roasted deer antler slices have the most significant effect on promoting the proliferation of bone marrow mesenchymal stem cells.

Key words: bone marrow mesenchymal stem cell, cell proliferation, antler slice, calcined, yellow clay, honey roasting, water extract, engineered stem cell

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