中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (5): 677-682.doi: 10.3969/j.issn.2095-4344.2016.05.012

• 心脏及血管损伤动物模型 Animal models of heart and vascular damage • 上一篇    下一篇

祛浊化痰中药干预食饵性动脉粥样硬化模型兔血脂及相关炎性因子的变化

宋筱靓，王 帅，王凤荣   

  1. 辽宁中医药大学附属医院，辽宁省沈阳市 110032
  • 收稿日期:2015-12-07 出版日期:2016-01-29 发布日期:2016-01-29
  • 通讯作者: 王凤荣，主任医师，教授，博士生导师，辽宁中医药大学附属医院，辽宁省沈阳市 110032
  • 作者简介:宋筱靓，男，1982年生，辽宁省沈阳市人，主治医师，主要从事中西医结合防治老年病研究。
  • 基金资助:
    辽宁省卫生计生委项目—动脉粥样硬化临床前期病变中医诊疗应用能力建设项目(LNCC-C01-2015)

Dispelling turbid phlegm herb affects the changes in serum lipid and related inflammatory factors in a rabbit model of dietary atherosclerosis

Song Xiao-liang, Wang Shuai, Wang Feng-rong   

  1. Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China
  • Received:2015-12-07 Online:2016-01-29 Published:2016-01-29
  • Contact: Wang Feng-rong, Chief physician, Professor, Doctoral supervisor, Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China
  • About author:Song Xiao-liang, Attending physician, Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China
  • Supported by:

     the Health and Family Planning Commission of Liaoning Province, No. LNCC-C01-2015

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摘要：

文章快速阅读：

文题释义：

动脉粥样硬化：是冠心病、脑梗死、外周血管病的主要原因。脂质代谢障碍为动脉粥样硬化的病变基础，其特点是受累动脉病变从内膜开始，一般先有脂质和复合糖类积聚、出血及血栓形成，进而纤维组织增生及钙质沉着，并有动脉中层的逐渐蜕变和钙化，导致动脉壁增厚变硬、血管腔狭窄。病变常累及大中肌性动脉，一旦发展到足以阻塞动脉腔，则该动脉所供应的组织或器官将缺血或坏死。由于在动脉内膜积聚的脂质外观呈黄色粥样，因此称为动脉粥样硬化。
祛浊化痰煎剂：源自仲景名方大柴胡汤，用药精简，立方主旨在“和”和“下”字，可和解与通下并行，针对邪入少阳、肝胆经气不利、三焦水道失调而引起痰瘀毒互结的有形实邪结聚之证，可和解表里、内泄热结，临床应用，功效卓著。

 

背景：多项动物实验和临床观察证明，祛浊化痰中药对血清脂质、脂蛋白和肝脂质有较好的调节作用。
目的：观察祛浊化痰中药对食饵性动脉粥样硬化兔单核细胞趋化因子1、C-反应蛋白及血脂的影响。
方法：将50只新西兰大白兔随机分为5组，空白对照组给予基础饲料喂养，同时灌胃给予生理盐水10 mL/(kg•d)，连续10周；模型组给予高脂饲料喂养制备动脉粥样硬化模型，同时灌胃给予生理盐水10 mL/(kg•d)，连续10周；祛浊化痰预防组给予高脂饲料喂养制备动脉粥样硬化模型，同时灌胃给予祛浊化痰中药10 mL/(kg•d)，连续10周；血脂康组给予高脂饲料喂养制备动脉粥样硬化模型，同时一次性灌胃给予血脂康10 mL/(kg•d)，连续10周；祛浊化痰治疗组给予高脂饲料喂养10周制备动脉粥样硬化模型，同时灌胃生理盐水10 mL/(kg•d)，连续6周，此后改为灌胃给予祛浊化痰中药10 mL/(kg•d)，连续4周。第10周末，检测兔血脂、C-反应蛋白、单核细胞趋化因子1 mRNA表达水平，以及取主动脉进行病理观察。
结果与结论：血脂康、祛浊化痰中药可通过降低血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、C-反应蛋白及单核细胞趋化因子1 mRNA表达水平，明显抑制动脉粥样硬化改变，其中祛浊化痰中药预防性应用的效果好于治疗性应用。 

ORCID: 0000-0002-8035-6714(王凤荣)

Abstract:

BACKGROUND: Many animal experiments and clinical observation proved that dispelling turbid phlegm herb has good adjustment function on serum lipid, lipoprotein and liver lipid.
OBJECTIVE: To observe the effects of dispelling turbid phlegm herb on monocyte chemotactic factor-1, C-reactive protein and serum lipids in rabbits with dietary atherosclerosis. 
METHODS: A total of 50 New Zealand white rabbits were randomly divided into five groups. Rabbits in the blank control group were fed with basic feed, and simultaneously intragastrically administrated physiological saline 10 mL/kg per day, for 10 consecutive weeks. Rabbits in the model group were given high-fat diet to prepare atherosclerosis models, and simultaneously intragastrically administrated physiological saline 10 mL/kg per day, for 10 consecutive weeks. Rabbits in the phlegm turbidity treatment group were given high-fat diet to prepare atherosclerosis models, and simultaneously intragastrically administrated dispelling turbid phlegm herb 10 mL/kg per day, for 10 consecutive weeks. Rabbits in the Xuezhikang group were given high-fat diet to prepare atherosclerosis models, and simultaneously administrated Xuezhikang 10 mL/kg per day, for 10 consecutive days. Rabbits in the phlegm turbidity treatment group were given high-fat diet for 10 weeks to prepare atherosclerosis models, and intragastrically administered physiological saline 10 mL/kg per day for 6 weeks, and then given dispelling turbid phlegm herb 10 mL/kg per day for 4 weeks. At 10 weeks, serum lipid, C-reactive protein, and monocyte chemokine 1 mRNA expressions were detected, and pathological observation of the aorta was performed.
RESULTS AND CONCLUSION: Xuezhikang and dispelling turbid phlegm herb could decrease serum total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipid protein cholesterol, C-reactive protein and monocyte chemokine 1 mRNA expression level, and apparently inhibited atherosclerotic changes. The preventive effect of dispelling turbid phlegm herb was better that its therapeutic effect.