中国组织工程研究

• 骨组织构建 bone tissue construction •    下一篇

阿仑膦酸钠改善去卵巢大鼠的骨基质结构

王  翔,郭海玲,赵咏芳,詹红生,石印玉   

  1. 上海中医药大学附属曙光医院石氏伤科医学中心,上海市中医药研究院骨伤科研究所,上海市  201203
  • 修回日期:2013-10-16 出版日期:2013-12-10 发布日期:2013-12-10
  • 通讯作者: 郭海玲,硕士,上海中医药大学附属曙光医院石氏伤科医学中心,上海市中医药研究院骨伤科研究所,上海市 201203 ghl22007190@163.com
  • 作者简介:王翔☆,男,1976年生,汉族,天津市人,2009年上海中医药大学毕业,博士,主治医师,主要从事中医药防治骨质疏松症的研究。 w8x@163.com
  • 基金资助:

     “中医骨伤科学”国家重点学科项目(100508)*;上海市中医药领军人才建设项目(2012-63-15)*;上海领军人才项目(041)*;“海派中医流派传承研究基地”项目(ZYSNXD-CC-HPGC-JD-001)*;国家自然科学基金项目(81073114,81001527,81102603,81173621)****;上海市科委重点科技攻关项目(11DZ1973401)*;国家教育部重点项目(212052)*;上海市教育委员会科研创新项目(12ZZ120)*;上海高校青年教师培养资助计划(szy11046)*;上海市卫生局青年科研项目(20114y004)*。

Alendronate improves bone matrix structure of ovariectomized rats

Wang Xiang, Guo Hai-ling, Zhao Yong-fang, Zhan Hong-sheng, Shi Yin-yu   

  1. Shi’s Center of Orthopedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Traumatology & Orthopedics, Shanghai Academy of TCM, Shanghai  201203, China
  • Revised:2013-10-16 Online:2013-12-10 Published:2013-12-10
  • Contact: Guo Hai-ling, Master, Shi’s Center of Orthopedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Traumatology & Orthopedics, Shanghai Academy of TCM, Shanghai 201203, China ghl22007190@163.com
  • About author:Wang Xiang☆, M.D., Attending physician, Shi’s Center of Orthopedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Traumatology & Orthopedics, Shanghai Academy of TCM, Shanghai 201203, China w8x@163.com

摘要:

背景:双膦酸盐可以提高骨密度、抑制骨吸收的作用已被临床所证实,但其对于骨骼基质结构的影响研究较少。
目的:实验通过观察双膦酸盐类药物——阿仑膦酸钠对骨结构及骨基质代谢的影响,探讨阿仑膦酸钠改善骨质量、提高骨强度的骨基质调控机制。
方法:实验建立去卵巢大鼠模型,用阿仑膦酸钠进行干预,同时设置模型组和假手术组进行对照。运用骨骼影像学、骨组织病理学和骨生物力学检测技术与酶联免疫吸附法观察阿仑膦酸钠对骨丢失大鼠骨密度、骨代谢、骨生物力学性能和骨结构的影响。
结果与结论:药物干预后4,8,12周,阿仑膦酸钠组骨密度均高于模型组(P < 0.05);药物干预8周后,与模型组相比,阿仑膦酸钠组骨代谢指标尿脱氧吡啶诺啉,血清Ⅰ型胶原羧基端前肽水平均降低(P < 0.05),腰椎和股骨的最大载荷、最大压强、模量以及Ⅰ型胶原不同交联形式的尿吡啶啉/脱氧吡啶啉值均增高(P < 0.05)。结果证实,阿仑膦酸钠能够对抗因雌激素缺乏导致大鼠骨量的丢失,提高生物力学性能,改善骨基质结构,同时恢复因去卵巢所导致的Ⅰ型胶原交联组分的改变。



中国组织工程研究
杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


全文链接:

关键词: 组织构建, 骨组织构建, 骨质疏松, 骨质量, 骨强度, Ⅰ型胶原, 交联, 双膦酸盐, 骨基质, 骨结构, 国家自然科学基金

Abstract:

BACKGROUND: Bisphosphonates that can increase bone density and inhibit bone resorption have been clinically confirmed, but the structure of the bone matrix has been less studied.
OBJECTIVE: To observe the effects of alendronate on bone structure and bone matrix metabolism, and then to investigate the controlling mechanism by which alendronate improves bone mass and increase bone intensity.
METHODS: An ovariectomized rat model was prepared and intervened with alendronate as treatment group. Model and sham-surgery groups were set as controls. Alendronate effects on bone mineral density, bone metabolism, bone biomechanics, and bone structure were observed in bone loss rats using bone imaging, bone tissue pathology and biomechanical test and enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: Alendronate intervention could fight against bone loss as compared with model group at weeks 4, 8, and 12 after treatment (P < 0.05). Compared with the model group, the expression of urinary deoxypyridinoline and serum carboxyterminal propeptide of type Ⅰ procollagen was decreased significantly after alendronate intervention (P < 0.05); the maximal load, maximal pressure and modelus on the lumbar vertebrae and femur were increased as well as ratio of urinary pyridinoline/deoxypyridinoline of type Ⅰ procollagen (P < 0.05). These findings suggest that alendronate intervention can inhibit bone loss in rats induced by estrogen deficiency, increase biomechanical properties, improve bone matrix structure, and meanwhile, recover the Ⅰ collagen crosslinking component due to ovariectomy.



中国组织工程研究
杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


全文链接:

Key words: osteoporosis, bone remodeling, ovariectomy, animals

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