中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (49): 8590-8595.doi: 10.3969/j.issn.2095-4344.2013.49.020

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

Neurogenin2基因调控许旺细胞转分化为神经元

许信龙,谢青松,潘红松,魏晓捷,陈再丰   

  1. 慈溪市人民医院神经外科,浙江省宁波市  315300
  • 修回日期:2013-09-08 出版日期:2013-12-03 发布日期:2013-12-03
  • 通讯作者: 谢青松,硕士,主治医师,慈溪市人民医院神经外科,浙江省宁波市 315300 qsxie@163.com
  • 作者简介:许信龙,男,1964年生,浙江省慈溪市人,汉族,1985年温州医学院毕业,主任医师,主要从事神经损伤与修复的基础和临床研究。 cry63929001@163.com
  • 基金资助:

    宁波市自然科学基金资助项目(2010A610070)*

Neurogenin2 gene-regulated Schwann cells differentiate into neurons

Xu Xin-long, Xie Qing-song, Pan Hong-song, Wei Xiao-jie, Chen Zai-feng   

  1. Department of Neurosurgery, Cixi Municipal People’s Hospital, Cixi  315300, Zhejiang Province, China
  • Revised:2013-09-08 Online:2013-12-03 Published:2013-12-03
  • Contact: Xie Qing-song, Master, Attending physician, Department of Neurosurgery, Cixi Municipal People’s Hospital, Cixi 315300, Zhejiang Province, China qsxie@163.com
  • About author:Xu Xin-long, Chief physician, Department of Neurosurgery, Cixi Municipal People’s Hospital, Cixi 315300, Zhejiang Province, China cry63929001@163.com
  • Supported by:

    Ningbo Natural Science Foundation, No. 2010A610070*

摘要:

背景:Neurogenin2(Ngn2)基因调控星形胶质细胞分化为神经元已被实验证实,这提示许旺细胞也可能通过基因调控分化为神经元。
目的:研究Neurogenin2基因调控大鼠许旺细胞向神经元分化的可行性。
方法:体外培养、纯化及鉴定大鼠许旺细胞,然后用含绿色荧光蛋白基因的慢病毒载体系统将Neurogenin2基因转染导入许旺细胞中,最后用含碱性成纤维细胞生长因子、表皮生长因子和脑源性细胞生长因子的无血清DMEM诱导培养许旺细胞2周。显微镜观察细胞形态,免疫细胞化学检测髓磷脂碱性蛋白及神经元特异性烯醇化酶。
结果与结论:许旺细胞转染导入Neurogenin2基因并诱导分化后,免疫荧光检测发现12.56%的细胞表达神经元标志性蛋白神经元特异性烯醇化酶,而对照组均未发现表达神经元标志性蛋白神经元特异性烯醇化酶。Neurogenin2基因植入可使大鼠许旺细胞表达神经元标志性蛋白神经元特异性烯醇化酶,提示该基因可调控许旺细胞转分化为神经元。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

关键词: 干细胞, 干细胞培养与分化, 许旺细胞, Neurogenin2, 基因, 分化, 神经元, 神经元特异性烯醇化酶, 干细胞图片文章

Abstract:

BACKGROUND: It is confirmed that astrocytes can differentiate into neurons by Neurogenin2 gene regulation, suggesting that Schwann cells may also differentiate into neurons by gene regulation.
OBJECTIVE: To evaluate the feasibility of Schwann cells differentiating into neurons by Neurogenin2 gene regulation.
METHODS: Rats Schwann cells were isolated, purified and identified. Then the Schwann cells were transfected with Neurogenin2 via green fluorescent protein gene-plentivirus. To induce neuronal differentiation, the Schwann cells were cultured in serum-free Dulbecco’s modified Eagle’s medium containing epidermal growth factor, basic fibroblast growth factor and brain-derived neurotrophic factor for 2 weeks. The morphology of induced cells was observed by microscope, and myelin basic protein and neuron-specific enolase were detected by immunocytochemistry.
RESULTS AND CONCLUSION: After transfection with Neurogenin2 via green fluorescent protein gene-plentivirus and induced differentiation, immunofluorescence assay demonstrated that 12.56% of the induced cell expressed neuron-specific enolase, but the control group did not express neuron-specific enolase. Neurogenin2 gene-transfected Schwann cells can express neuron-specific enolase, suggesting Neurogenin2 gene may regulate transdifferentiation of Schwann cells into neurons.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

Key words: Schwann cells, nerve regeneration, neurons, epidermal growth factor, fibroblast growth factors, cell differentiation, genes

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