中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (50): 9311-9315.doi: 10.3969/j.issn.2095-4344.2012.50.001

• 组织构建与中医药 tissue construction and traditional Chinese medicine •    下一篇

苗药九仙罗汉接骨汤干预胫骨干骨折不同时相点的X射线比较

杨彦君1，熊 屹2，程志刚2，易洪成2，吕 英2，唐良华2   

  • 出版日期:2012-12-09 发布日期:2012-12-09

Miao Nationality’s Jiuxianluohanjiegu decoction promotes fracture healing: X-ray comparison at different phases

Yang Yan-jun1, Xiong Yi2, Cheng Zhi-gang2, Yi Hong-cheng2, Lü Ying2, Tang Liang-hua2   

  • Online:2012-12-09 Published:2012-12-09

PDF

627

被引次数

6

摘要：

背景：九仙罗汉接骨汤由仙桃草、九节茶、地罗汉三味苗药组成，具有活血散瘀通络，接骨消肿止痛之功效。
目的：观察苗药九仙罗汉接骨汤促进兔胫骨干骨折愈合的作用及机制。
方法：采用传统中药方法煎煮九仙罗汉接骨汤，制备成实验所需浓度的煎剂，微孔滤器过滤除菌备用。新西兰大白兔右胫骨前部中断造成宽3 mm深2 mm的骨缺损模型，随机分为接骨汤组、三七对照组和生理盐水组，分别灌胃给药。通过不同时相点的新西兰大白兔的体质量、体温变化，X射线、白细胞、红细胞，血小板，血钙，血磷，碱性磷酸酶等的浓度变化观察骨折愈合情况。
结果与结论：九仙罗汉接骨汤促进骨痂生长，缩短骨折愈合时间。接骨汤组与对照组两两比较，体质量增长快，血清钙、血清磷明显升高并持续2周，血清碱性磷酸酶明显升高并持续21 d(P < 0.05)，可以促进钙盐在骨折断端的沉积，对骨折愈合有明显的促进作用，利于骨折愈合。提示苗药九仙罗汉接骨汤对兔胫骨干骨折有促进其骨痂生成，缩短骨折愈合时间的作用。

Abstract:

BACKGROUND: Jiuxianluohanjiegu decoction composed of purslane speedwell herb, glabrous sarcandra herb, and Glomeris nipponica Kishida can promote blood circulation to clear blood stasis and remove obstruction in the collateral, rejoin bone fracture, reduce selling, and relieve pain.
OBJECTIVE: To observe the effect and mechanism of Miao Nationality’s Jiuxianluohanjiegu decoction on promoting the healing of rabbit tibial fractures.
METHODS: Jiuxianluohanjiegu decoction was decocted to the required concentration using the traditional Chinese medicine method, and then filtrated using millipore filtration for standby. Right tibialis anterior disruption in New Zealand rabbits, 3 mm in width and 2 mm in depth, was performed to prepare bone defect models. Then, the models were randomly divided into three groups: Jiegu decoction group (experimental group), Sanqi (Panax Notoginseng) control group, and saline group. Body weight, body temperature, X-ray, with blood cells, red blood cells, platelets, serum calcium, serum phosphate, alkaline phosphatase concentration changes of New Zealand rabbits were observed at different phases of fracture healing.
RESULTS AND CONCLUSION: Jiuxianluohanjiegu decoction promoted callus growth, and shortened the healing time. Compared with the other two groups, weight growth were faster, serum calcium and phosphorus levels were significantly increased for 2 weeks, and serum alkaline phosphatase level was increased significantly for 21 days in the experimental group (P < 0.05). Jiuxianluohanjiegu decoction could promote calcium deposition at the fracture end, and greatly contribute to fracture healing. These findings indicate that Miao Nationality’s Jiuxianluohanjiegu decoction can promote callus formation and shorten healing time in the treatment of rabbit tibial fractures.