中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (30): 5543-5546.doi: 10.3969/j.issn.1673-8225.2011.30.009

• 人工假体 artificial prosthesis • 上一篇    下一篇

假体磨损颗粒钴铬离子影响成骨细胞增殖及RANKL、骨保护素的表达

李俊宁，袁晓军，徐文华，傅云根，曹盛生   

  1. 宜春市人民医院骨二科，江西省宜春市  336000
  • 收稿日期:2011-03-27 修回日期:2011-05-11 出版日期:2011-07-23 发布日期:2011-07-23
  • 通讯作者: 袁晓军，硕士，宜春市人民医院骨科，江西省宜春市336000 YXJ7491@163.com
  • 作者简介:李俊宁★，男，1969年生，江西省宜春市人，汉族，2010年南昌大学医学院毕业，硕士，副教授，主要从事人工关节基础研究与应用。 junning1964@163.com

Proliferation of mouse osteoblasts and expression of receptor activator of nuclear factor kappa B ligand and osteoprotegerin induced by cobalt and chromium ions

Li Jun-ning, Yuan Xiao-jun, Xu Wen-hua, Fu Yun-gen, Cao Sheng-sheng   

  1. Department of Orthopedics, the People’s Hospital of Yichun City, Yichun  336000, Jiangxi Province, China
  • Received:2011-03-27 Revised:2011-05-11 Online:2011-07-23 Published:2011-07-23
  • Contact: Yuan Xiao-jun, Master, Department of Orthopedics, the People’s Hospital of Yichun City, Yichun 336000, Jiangxi Province, China YXJ7491@163.com
  • About author:Li Jun-ning★, Master, Associate professor, Department of Orthopedics, the People’s Hospital of Yichun City, Yichun 336000, Jiangxi Province, China junning1964@163.com

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277

被引次数

2

摘要：

背景：金属-金属假体置入体内后可以发生腐蚀或磨损，释放镍、钴、铬、钛等金属离子，诱导局部炎性因子的释放。
目的：观察Co2+、Cr3+对小鼠成骨细胞增殖的影响，以及成骨细胞暴露在Co2+ 、Cr3+条件下RANKL、骨保护素基因的表达。
方法：体外培养成骨细胞，实验分两组，对照组给予生理盐水，离子组给予钴、铬离子干预。
结果与结论：显微镜直接计数法显示干预后1~6 d对照组随时间推移细胞数目显著增加，离子组则增加不明显。共培养24，48 h后，RT-PCR结果显示离子组RANKL、骨保护素基因表达较对照组均增加，以RANKL增加更显著(P < 0.05)，RANKL/OPG mRNA的比率也明显增加。提示金属离子对成骨细胞的增殖有显著抑制作用，且可刺激成骨细胞RANKL、骨保护素mRNA的表达。

关键词: 钴离子, 铬离子, 成骨细胞, RANKL, 骨保护素

Abstract:

BACKGROUND: Metal-metal prosthesis can appear with corrosion or wear, and release nickel, cobalt, chromium, titanium, and some inflammatory factors locally.
OBJECTIVE: To investigate the effects of cobalt (Co2+) and chromium (Cr3+) ions on the proliferation of mouse osteoblasts and expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG).
METHODS: Osteoblasts cultured in vitro were divided into two groups: control group with normal saline and ion group with cobalt and chromium ions.
RESULTS AND CONCLUSION: The number of cells in the control group was increased remarkably at 1-6 days after intervention, while no changes occurred in the ion group. RT-PCR results showed that the expression of RANKL and OPG was increased as compared with control group at 24 and 48 hours after coculture, especially RANKL expression (P < 0.05). The ratio of RANKL/OPG mRNA was also improved. Co2+ and Cr3+ ions have the remarkable inhibitory action on osteoblast proliferation and can stimulate the expression of RANKL and OPG mRNA from osteoblasts.

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