中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (29): 5359-5362.doi: 10.3969/j.issn.1673-8225.2011.29.010

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

重组人类骨形态蛋白2复合自固化磷酸钙材料在体内的血管化

王  炜1，玛依拉•吾甫尔2，阿不都赛米•艾买提1，艾合买提江•玉素甫1   

  1. 新疆医科大学第一附属医院，1骨科中心显微修复外科，2心脏中心，新疆维吾尔自治区乌鲁木齐市   830054
  • 收稿日期:2011-04-01 修回日期:2011-05-12 出版日期:2011-07-16 发布日期:2011-07-16
  • 通讯作者: 艾合买提江?玉素甫，教授，博士生导师，新疆医科大学第一附属医院骨科中心显微修复外科，新疆维吾尔自治区乌鲁木齐市 830054 ahmatjang@163.com
  • 作者简介:王炜★，男，1971年生，甘肃省武威市人，汉族，新疆医科大学在读硕士，主要从事创伤修复重建方面的研究。 Wangwei201107@yahoo.cn
  • 基金资助:

    国家自然科学基金(30960390)及新疆医科大学一附院科研专项基金(2007-GXB-04)资助。

Vascularization of autosetting calcium phosphate cultivated with recombinant human bone morphogenetic protein 2

Wang Wei1, Mayila•Wufuer2, Abudusaimi•Aimaiti1, Aihemaitijiang•Yusufu1   

  1. 1Department of Microsurgical Repair, Orthopedics Center, 2Heart Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-04-01 Revised:2011-05-12 Online:2011-07-16 Published:2011-07-16
  • Contact: Aihemaitijiang? Yusufu, Professor, Doctoral supervisor, Department of Microsurgical Repair, Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China ahmatjang@163.com
  • About author:Wang Wei★, Studying for master’s degree, Department of Microsurgical Repair, Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Wangwei201107@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30960390*; Specific Research Fund of the First Affiliated Hospital of Xinjiang Medical University, No. 2007-GXB-04*

PDF

300

被引次数

5

摘要：

背景：深筋膜瓣促进组织工程骨血管化是一种成熟的技术，但动物种属不同、植入材料不同均会使血管化的结果产生较大的差异。
目的：比较复合重组人类骨形态蛋白2的自固化磷酸钙人工骨和单纯的自固化磷酸钙人工骨在比格犬带蒂筋膜瓣内的血管再生能力。
方法：分别将复合重组人骨形态蛋白2的自固化磷酸钙人工骨和单纯的自固化磷酸钙人工骨包裹于12只成年比格犬腰背部两侧带蒂深筋膜瓣中，于术后第2，4，8，16周各随机选取3只动物摘取血管化标本，进行大体观察、苏木精-伊红染色、Masson染色及血管内皮细胞Ⅷ因子相关抗原(ⅧRAg)免疫组织化学染色观察比较新血管的生成情况。
结果与结论：在材料植入后4周、8周和16周，两组均发现带蒂筋膜与植入材料接触区出现明显血管化，并随时间延长，向材料内部延伸；实验组相对血管面积百分比和阳性染色的面密度值在2，4，8，16周时均高于对照组。结果显示两种人工骨在筋膜内均有一定的血管化能力，但复合重组人骨形态蛋白2的自固化磷酸钙人工松质骨在体内的血管化较单纯自固化磷酸钙人工松质骨更为明显。

关键词: 血管化, 自固化磷酸钙, 筋膜瓣, 重组人类骨形成蛋白2, 比格犬

Abstract:

BACKGROUND: Deep fascia promotes vascularization of tissue-engineered bone, which is a mature technology, but different animal species and different implant materials can result in a great difference in vascularization.
OBJECTIVE: To compare the vascularization of autosetting calcium phosphate cement cultivated with recombinant human bone morphogenetic protein 2 (rhBMP-2) and a single autosetting calcium phosphate cement implant in beagle dogs with deep fasica flaps.
METHODS: Twelve healthy adult beagle dogs were respectively implanted with autosetting calcium phosphate cement cultivated with rhBMP-2 in the left thigh (experimental sides) and a single autosetting calcium phosphate cement in right thigh (control sides). The vascularization in each condition was assessed by experiment study (Physical, Massion stain, the hematoxylin-eosin stain, ⅧRAg marked) at time intervals of 2, 4, 8 and 16 weeks after operation.
RESULTS AND CONCLUSION: In experimental groups and in control groups, vascularization was found. New vessels invaded in scaffold with time. In experimental groups, the amount of vessels and the expression of ⅧRAg were stronger than those in control groups at 2, 4, 8, and 16 weeks. The deep fasica flaps have great effect on the vascularization. The deep fasica flap binding with rhBMP-2 is proved to be the better in vascularization of autosetting calcium phosphate cement.

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